Lonza Bioscience Blog

How to quickly detect off-targets when performing experiments with CRISPR/Cas9 technology.

These zombie cells are known to reduce proliferation capacity and gradually decay culture.

Learn more about the clinical trials: What are they exactly? What types of trials are there? How many phases are there? What happens in each phase?

Read a story our Sales Team shared recently that touched our hearts and shows why we love our jobs at Lonza Bioscience.

R.I.P, RPT. Hello, MAT! 

Genome-editing with 4D-Nucleofector^® System was recently used to generate new cellular models for Parkinson’s disease. 

Learn about genome editing in school. Dr. Martie Verschuren’s article is about teaching novel technologies for genome editing and how to keep students up to date in this exiting field of technology.

This article summarizes the role glutamine plays in cell culture and describes benefits of using L-alanyl-Glutamine versus standard L-glutamine.

Learn more about a real-life endotoxin test case

A New Sample Testing Guide

Get information about factors to consider when selecting cells to study COVID-19 transmission.

PBMC collected from patients were reprogrammed into iPSC by Nucleofection. Generated IPSCs still carry the mutation causing the disease.

Cell and gene therapy are a hot topic, but are you aware of the requirements around endotoxin testing for these products? Here is a short overview. 

A recent published application note is showing the transfection results of cryopreserved human hepatocytes with different substrates and keeping also an eye on functionality e.g. CYP enzymes.

Are you curious to know some details?

As recombinant methods get more and more attention and are also more and more considered/ implemented by pharmacopoeias, a new review addresses the comparability of results with recombinant methods to the traditional horseshoe crab blood based methods. Jay Bolden and co-workers did an extensive study of peer-reviewed literature to find publications where naturally contaminated samples were tested side by side (same samples, only difference being the test method, i.e. rFC vs LAL/TAL). Spoiler: both types of assays seemingly give very similar results and are comparable in protecting patient safety.

For many experiments it is important to determine and know how many cells you have. Using a hemocytometer is rather basic and works well when done correctly. 

Lonza is bridging the gap from rabbit pyrogen testing to in vitro detection of pyrogens and provides another sustainable method for endotoxin and pyrogen detection to the market. 

This article summarizes the process of cryopreservation and includes tips and tricks to improve freezing methods. 

This blog post briefly describes the process that customers will follow to set up a product reserve.

Identifying potential treatments for COVID-19 is of utmost importance right now. This blog post highlights some recent work done for high throughput screening of drug candidates for SARS-CoV-2 using software designed for phenotypic analysis. 

With the publication of supplement 10.3, EP officially introduces recombinant Factor C as a compendial method.

The follow article will summarize the therapeutic properties of MSCs, quality attributes, expansion methods, as well as some major challenges these cells create when used in cell therapy applications. 

Software Beyond the Basics.

Explore the history of LAL endotoxin testing towards the development of the recombinant Factor C assay. The American horseshoe crab population is now a vulnerable species, just one step from being endangered. The rFC assay has gone a long way to be accepted but as there is a growing recognition of its importance by various Pharmacopeia, a widespread adoption of the rFC method could be on the horizon.

Live cell imaging performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min) to capture CD34+ cell division.

How to handle the natural killers for genomic engineering purposes?

Learn from the authors about the potential overcome of some limitation of NK cell-based therapies and check for the detailed protocol of Cas9 RNP transfection. 

Maybe it is also a start for you? 

Can tolerogenic dendritic cells help to restore tolerance in autoimmune diseases?

A novel T cell: PMBC assay to test for immunogenicity.

What’s the point about HLA status and why is it important in research?

For their essential function in catalyzing the adaptive immune response, dendritic cells are more and more exploited in tumor immunotherapies. In this context, they are often used in vitro screens. Thus, a reliable method for their transfection became evident. Here, we propose an efficient way to achieve good transfection results in terms of viability and efficiency using Nucleofection^TM Technology.

The development of a reproducible 3D in vitro model of tumour growth.

The Nucleofection^TM Technology used for generation of three SCD-iPS and three control iPS cell lines.

CAR-T therapy is a new cutting edge treatment involving modification of human T cells, but also has some challenges to overcome. Learn more about it in a brief overview of this therapy.

This article highlights a few key hallmarks in vaccine development.

During the last session of the European Pharmacopoeia Commission, endotoxin testing with rFC was adopted as general chapter 2.6.32. 

Learn more about data integrity and how automation can help you there in endotoxin testing.

This article summarizes the major elements of the protein production process.

Different techniques for genome editing in diverse cells and species.

Best wishes for 2020 - Your Scientific Support Team USA and Europe/Asia/Pacific.

USP just released a new guidance chapter to provide supporting information on the endotoxins test.

As part of the European Endotoxin Seminar series Lonza recently held a 2 days workshop in Manchester.

Electro-pulse stimulation is used in vitro to study the response of human-mouse hybrid myotubes.

Different aspects of the endotoxin testing are considered to shed some light on articles that question the use of rFC for release testing.

Islet-like structures generated in vitro from adult human liver stem cells revert hyperglycemia in diabetic SCID mice.

As part of the European Endotoxin Seminar series Lonza recently held a 2-days workshop in Cologne.

This blog post discusses human hepatic stellate cells, which are intrinsically involved in liver damage, and I highlight a key citation that may help study liver damage using cryopreserved human cells rather than animal liver models.

Re-activation of fetal hemoglobin expression by CRISPR-Cas9 for treatment of beta-hemoglobinopathies

The authors describe a novel approach that leads to higher editing efficiency in your CRISPR experiments.

If you are a researcher or new to the LAL assay, testing serum or plasma samples for endotoxin may seem a daunting task. Our Technical Tips will help you.

Light exposure causes photoxidation which can generate harmful, highly reactive species that are damaging to your culture.

Improving the cultivation of CD19.CAR-T cells transfected with piggyBac system is a step towards clinical use.

Efficient CRISPR RNP genome editing of human Natural Killer cell using the 4D-Nucleofector^TM Device

Endotoxin recovery study with 4 different types of plastic sample containers.

Sometimes it’s the basics that can make or break an experiment and Nucleofection^TM experiments are no different. Cell handling, proper culture conditions, and following the protocol are all critical. Here are some important things to keep in mind when performing your own Nucleofection^TM experiments.

Discovering the impact of GI-tract microbiome-generated neurotoxin on NF-L mRNA in the Alzheimers Brain

Lonza offers a guideline how to screen plastic accessories to be used with Bacterial Endotoxin Testing (BET)

Cell culture is expensive and time consuming. Effective technique and protocols can help keep cells healthy in culture. These are some common errors to avoid when culturing primary cells. 

Helping the crabs while helping yourself.

Are you investigating Low Endotoxin Recovery (LER)? PDA has released its Technical Report 82 on this topic - learn what it's all about!

Scientists elucidating the mechanisms of the R47H Trem2 variant discover they cannot be replicated in humans

Review of "CRISPR-based Genetic Screen in Physiological Relevant Primary Cells" from Judd Hultquist.

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