Using Longer Single Guides to Increase Editing Efficiency

CRISPR has quickly become the method of choice for genome editing applications. Its simple concept and seemingly endless potential drive researchers to search for methods to improve their results. Everyone wants higher editing efficiency, less errors, and more specificity. Can that be achieved easily? I was looking for answers when I found the paper that I present you with today.

The authors describe a unique approach where using longer single-guides (sgRNA’s) they are able to achieve higher editing efficiency. I certainly found this interesting and I thought you would as well.

I wish you good luck in all of your future experiments.

Written by Sean

Scientific Support Specialist, Lonza Pharma-Bioscience Solutions at Lonza

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Using Longer Single Guides to Increase Editing Efficiency

CRISPR screen of SAECs with 384-well Nucleofector® System

CRISPR off-target effects

CRISPR/Cas9 based genome engineering of NK cells using Nucleofector® Technology

Need technical data?

CRISPR/Cas9 based genome engineering of NK cells using Nucleofector^® Technology