Primary cell culture provides more biologically relevant data than that generated using cell lines. Concerns over the use of cell lines have resulted in a growing need for primary cells in a variety of applications from basic research to drug discovery. Often, primary cells are combined with newer technologies such as 3D cell culture given a recent surge within the research community to use better reagents to improve research.
Primary cells are isolated directly from the donor tissue and therefore maintain tissue characteristics that are closer to in vivo conditions. Although in theory primary cells can be sourced from any tissue type, isolating them, characterizing them and maintaining them successfully in culture requires substantial expertise, infrastructure and access to the appropriate, ethically sourced donor tissue type. Researchers who choose to isolate fresh cells often face obstacles of unsuccessful isolations, comparing data with off-site groups, or comparing data over a long-term study due to the variability among the reagents and the procedures used to isolate cells. As a result, commercially available options are sought by researchers, where available, to reduce workload of building cell culture reagents and instead focus their efforts towards research applications. When cross collaborating with other groups or sites, purchased cryopreserved cells from trusted commercially available sources allow researchers to work with the same batch across multiple sites. This can potentially reduce donor-to-donor or manufacturing variability across experiments.
For more than 30 years, researchers have been relying on Lonza for consistent, high-performing products and outstanding scientific support team. Lonza offers a comprehensive collection of primary cells and media products supporting many research areas. All our human and animal primary cells have been ethically sourced and authenticated by thorough QC testing.