For the transfection of primary cells and cell lines using the NucleofectorTM Technology, transfection optimization is usually not required. Lonza provides ready-to-use, cell type-specific Optimized Protocols for more than 150 cell types. In addition, our Knowledge Database contains transfection data for close to 1500 cell types. To determine if an Optimized Protocol is available for your cell type of interest, search our Knowledge Database or contact the Scientific Support Team.
However, if no cell type-specific protocol is available for your cell type of interest, transfection optimization is a simple, straightforward process.
Please select the explanation applicable to your current situation by clicking on the bar below in order to determine the best optimization strategy for your cell type.