The PyroGeneTM Recombinant Factor C Assay is the evolution of endotoxin detection testing. Based on the activation of a recombinant form of Factor C, the first component in the horseshoe crab clotting cascade activated by the presence of endotoxin, the recombinant Factor C assay offers the same reliability as an LAL method – without the use of animal resources. The recombinant Factor C method works through a single enzymatic step compared to the multiple step enzymatic process necessary for LAL assays.
Learn more about our rFC assay by visiting the PyroGeneTM Recombinant Factor C Assay product page where you can find comprehensive details, including a product overview, educational materials, and scientific data about the effectiveness of rFC as a BET assay.
In the PyroGeneTM rFC assay, activated Factor C directly cleaves a fluorogenic substrate, producing a signal that is read using a standard fluorescent plate reader. Due to the high dynamic range of the fluorescent signal, PyroGeneTM rFC delivers a quantitative range of 5.0 EU/ml – 0.005 EU/ml in a single step, with better resolution than conventional kinetic LAL assays.
The PyroGeneTM rFC method is a quantitative assay that is run on a 96-well plate incubated in a microplate reader at 37°C that measures fluorescence with excitation/emission wavelengths at 380/440 nm. In the presence of endotoxin, activated rFC will cleave the fluorogenic substrate, causing the solution to fluoresce.
The graph on the left illustrates the enzymatic cascade of the recombinant Factor C assay compared to the enzymatic activation pathway of traditional LAL assays, like the kinetic chromogenic and kinetic turbidimetric assays.