Humanized mice from CD34+ cells and PBMCs

Primary cells for humanized mice

Humanized mice («humice») are mice in which human cells (or genes, tissues, and organs) have been introduced in order to represent a functional model that more closely resembles that of human biology. The use of humanized mice in critical disease research areas has been expanding significantly over the past decade, including

Immuno-oncology
Infectious disease
Transplantation research
Graft vs host disease (GVHD)
T cell modulation

Additionally humanized mice provide valuable models for safety testing including ADME/TOX and Immunogenicity impact.

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Generating humanized mice

There are different types of humanized mice, each with specific relevance to a research application. Two of the most common and well used humanized mice are CD34 humanized mice and PBMC humanized mice. These models are generated by injecting human immune cells into an immune compromised mouse in order to reconstitue a functional human immune system.

CD34+ humanized mice

Applicable for long term (up to 12 month) studies
Reconstitution in the mice takes ~12 weeks
Cord blood CD34+ cells preferred due to efficient engraftment
Robust T cell maturation and T cell dependent inflammatory responses
Example uses: Immuno-oncology, Infectious disease, Immunogenicity of biologics

PBMC humanized mice

Applicable for short term studies
Reconstitution in the mice takes a few days
Functional adult immune cells (low numbers of B cells)
Example uses: GvHD studies, immuno-oncology, HIV infection

Humanized Mice Illustration

Humanized mice with CD34+ AND PBMCs

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Our advantage

With Lonza’s 30+ years of experience in primary cell isolation and tissue acquisition, our CD34 cells and PBMCs are ideal for use in generating humanized mice, and have been used by leading suppliers for years. Cryopreserved cells are immediately available in multiple sizes, and with a wide donor variety.

CD34+

Catalog#	Description
2C-101	Cryopreserved human cord blood CD34+ cells , 1M
2C-101A	Cryopreserved human cord blood CD34+ cells , 0.5M
2C-101B	Cryopreserved human cord blood CD34+ cells , 0.1M
4Y-101C	Cryopreserved human CD34+ cells from mobilized blood, 1M
2C-101H	Cryopreserved human cord blood CD34+ cells, ≥ 1 million cells with HLA information
2C-101HX	Cryopreserved human cord blood CD34+ cells ≥ 2 million cells with HLA information

PBMCs

Catalog#	Description
CC-2702	Cryopreserved human peripheral blood mononuclear cells (PBMCs), 50M
4W-270	Cryopreserved human peripheral blood mononuclear cells (PBMCs), 10M
4W-270A	Cryopreserved human peripheral blood mononuclear cells (PBMCs), 25M
4W-270C	Cryopreserved human peripheral blood mononuclear cells (PBMCs), 100M