The Journal of Visualized Experiments (JoVe) shows a very helpful application video for genome editing of several different cells are organisms. Farboud et al. explain effective and adaptable techniques and a straightforward protocol for precise genetic manipulation with the CRISPR (clustered regularly interspaced short palindromic repeats) Cas9 RNP (CRISPR associated ribonucleotide protein). The authors show different methods for different species. The video starts with Nucleofection using the 4D-NucleofectorTM X Unit for co-transfection of the CRISPR/Cas9 complexes into human primary T cells and mobilized hematopoietic CD34+ stem cells (HSPCs). It highlights important steps when you mix the complexes and shows useful tips for successful experiments. The group from California shows in the second part of the video the genetic manipulation of entire organisms like the roundworm Caenorhabditis elegans and the crustacea Parhyale hawaiensis with microinjection methods.
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Video protocol for CRISPR/Cas9 genome editing