In contrast to LAL, the monocytes, which are the key cells of innate immunity, respond not only to endotoxin but to non-endotoxin pyrogens (NEP) in general. The immune response is the production of cytokines such as IL-6 during a stimulation period. IL-6 offers the advantage of being fully secreted. Therefore, the cell culture supernatant containing the released IL-6 cytokines can be easily analyzed with a traditional ELISA assay.
For your convenience, we offer all components that enable you to perform the complete procedure:
- PyroCellTM MAT Cells & Supplement: Sufficient for 3 plates
- PeliKine Compact IL-6 Kit: Antibodies, reagents, and plates (ELISA kit)
- PeliKine Tool Set: Buffers needed for the ELISA, included in the ELISA kit