Speeding up the PyroCell® MAT Testing

The monocyte activation test (MAT) is an in-vitro test system used to detect pyrogens in pharmaceutical preparations. It serves as replacement of the rabbit pyrogen test (RPT) that is consuming experimental animals. As compared to the Bacterial Endotoxin Test (BET) the MAT is capable of detecting non-endotoxin pyrogens in a sensitive manner.

The PyroCell^® MAT System consists of cryopreserved pooled of human peripheral blood mononuclear cells (PBMC), an optimized supplement either based on fetal bovine serum (FBS) or human serum (HS), and a human IL-6 ELISA.

The Rapid ELISA Kit comprises two kit boxes, i.e. the PeliKine IL-6 ELISA Rapid Set A, containing binding antibodies, IL-6 reference standard and the HPE dilution buffer and the Pelikine IL-6 ELISA Rapid Set B (formerly known as Pelikine Toolset) that now includes the pre-coated plates, buffers, substrate and stop solution.

The new PeliKine human IL-6 Rapid ELISA assay is validated with the PyroCell^® MAT Kit and PyroCell^® MAT HS Kit for comparable performance. The two kits are available side-by side until Q1 2024. The performance report comparing the two ELISA kits and the customer notification letter can be retrieved upon request. Please reach out to scientific.support.eu@lonza.com (for Europe, Africa, and APAC) or to scientific.support@lonza.com (for AMERICAS).

Written by Ingo

Senior Scientific Support Specialist