NHBE - Human Bronchial/Tracheal Epithelial Cells for B-ALITM Culture
NHBE - Human Bronchial/Tracheal Epithelial Cells for B-ALITM Culture
Cryopreserved Normal Human Bronchial Epithelial (NHBE) cells, screened and guaranteed for differentiation, in B-ALITM (Bronchial Air Liquid Interface) cell culture.
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Product Overview
Normal and Diseased Bronchial Epithelial (NHBE and DHBE) cells are isolated from epithelial lining of airways above bifurcation of the lungs. CC-2540S are NHBEs suitable for air-liquid interface cell culture and are guaranteed for differentiation marked by cilia formation, mucin production, and TEER levels.
All Cryopreserved human bronchial epithelial cells are guaranteed through 15 population doublings. Cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.
Select Applications with Lonza's Human Bronchial Epithelial Cells
- A Rotating Bioreactor for Scalable Culture and Differentiation of Respiratory Epithelium”. Respiratory epithelium is difficult to grow as it requires a well-maintained polarizing air‐liquid interface (ALI) to maintain differentiation. Raredon et al. describe a scalable and easy method to conduct air-liquid interface with Lonza's bronchial epithelial cells guaranteed for ALI differentiation (Cat. No. CC-2540S).
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Storage and Content
1 x Cryopreserved ampule containing ≥500,000 cells
SDS, CoA, and Instructions
Safety Data Sheets (SDS)
Choose a language to view the SDS.
Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
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Instructions - Airway Epithelial Cell Systems
Instructions for Proper Use and Culture of Airway Epithelial Cells -
Primary Cells and Media Reference Guide
A comprehensive collection of Lonza primary cell and media products supporting many research areas. -
Instructions - B-ALI™ Air-Liquid Interface Medium
Instructions for Proper Use and Culture -
B-ALI Immune-Airway Co-Culture Model: Normal Human Bronchial Epithelial cells and Peripheral Blood Mononuclear Cells - Instructions for use
Protocol to culture B-ALI NHBE cells with PBMCs
Educational Material
Brochures, White Papers etc.
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B-ALI Bronchial Air Liquid Interface Media Kit, a Guaranteed 3D in vitro Model for Respiratory Research
Lonza Whitepaper -
Primary Human Airway Cells and Media
Airway Cell and Media Reference Guide -
Gene Expression Differences in Airway Epithelial Cells from Asthma or COPD Donors
Access to diseased primary cells from patients diagnosed with COPD or asthma provides a convenient, biologically relevant model to assess genetic and in some cases phenotypic changes from normal, healthy donor tissues. In this study, we grew human bronchial epithelial (HBE) cells from normal, COPD-, and asthma-diagnosed human donor lung tissues in an air-liquid interface (ALI) model to assess potential phenotypic changes in vitro as well as provide a differentiated layer of cells to harvest for gene expression studies using the StellARray™ Gene Expression System. -
Primary Cells and Media Reference Guide
A comprehensive collection of Lonza primary cell and media products supporting many research areas. -
B-ALI Bronchial Air Liquid Interface Media Kit, a Guaranteed 3D in vitro Model for Respiratory Research
CD-SP095_BALI-Bronchial-Air Liquid-Interface_sec.pdf -
Primary Cells for Airway Research – Infographic
Infographic showing how Lonza primary cells can aide in your airway research -
Primary Human Hepatocyte 3D Spheroids Co-cultured with Non-Parenchymal Cells
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Modeling Lung Inflammation in 3D
A Physiologically Relevant Model for Studying Interactions Between Immune Cells and the Lung Epithelium Using Lonza's Bronchial Air-Liquid Interface (B-ALI™) System
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