P3 Primary Cell 384-well NucleofectorTM Kit
The P3 Primary Cell 384-well NucleofectorTM Kit is one of our five kits suited for high-throughput transfection of primary cells when working with the 384-well HT NucleofectorTM System.
This kit has been determined to be optimal for transfecting following cell types:
- B cell (human)
- CD34+ cell (human)
- Chondrocyte (human)
- DC (human)
- DC (mouse), mature
- ES (human), H9
- ES (mouse)
- Hepatocyte (human)
- HMEC (human)
- Keratinocyte (human)
- Macrophage (human)
- Monocyte (human)
- Neuron (rat), brain Hi/Cx
- NHBE (human)
- RPE (human)
- T cell (human), stim.
- T cell (mouse), unstim.
The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
The kit is available in different variants for either 2 x 384 or 10 x 384 reactions.
- High transfection efficiencies for primary cells
- Cells maintain functionality post transfection
- Seamless integration into automated liquid handling environments
- Conditions are transferable between 4D-NucleofectorTM System, 96-well ShuttleTM System and 384-well NucleofectorTM System
- Transfection of low cell numbers down to 2 x 104 primary cells
- High-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR libraries
Content & Storage
22.5 mL P3 Primary Cell NucleofectorTM Solution
5 mL Supplement 1
50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
2 x 384-well NucleocuvetteTM Plate (20 μl)