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384-well Nucleofector® System

Catalog #: AAU-1001
An independent Nucleofector® Platform for high-throughput transfection in 384-well format

Product Overview

The 384-well Nucleofector® System is an independent platform for high-throughput Nucleofection in a 384-well format. With an extremely fast plate processing time of 1 minute per plate, it is perfectly suited for screening applications with maximum reproducibility.

The 384-well Nucleofector® System consists of a Power Supply Unit generating the high voltage pulses, a Plate Handler Unit and an intuitive PC-based Operation Software.

The 384-well Nucleofector® Kits use existing 4D-Nucleofector® 96-well Unit Protocols with conductive polymer 384-well Nucleocuvette® Plates.

There are cell type-specific Optimized Protocols or recommendations available in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.


Technical Specifications

Dimensions (w × d × h)

  • 384-well Nucleofector® Plate Handler:  40 cm × 42 cm × 15 cm (15.7 × 16.5 × 5.9 in)
  • 384-Nucleofector® Power Supply: 13.5 cm × 50 cm × 45 cm (5.3 × 19.6 × 17.7 in) 


  • 384-well Nucleofector® Plate Handler: 10 kg (22.04 lb)
  • 384-well Nucleofector® Power Supply: 14 kg (30.86 lb)

Laptop operating system: Windows 7 or 10


  • Fast - Processes a 384-well plate in one minute, carousel capable of handling two plates 
  • High performance combined with minimum material consumption - Nucleofection of low cell numbers down to 2x104 cells 
  • Easy-to-use -  Uses existing 4D-Nucleofector® 96-well Unit Protocols 
  • Automation compatible - Seamless integration into automated liquid handling environments


  • High-throughput Nucleofection of low cell numbers down to 2 × 104 cells
  • Screening of arrayed cDNA, RNAi or CRISPR libraries


Content & Storage


1 HT Nucleofector® Plate Handler
1 HT Nucleofector® Power Supply
1 Laptop with HT Nucleofector® Software


Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

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Demo Request

Nucleofection in 96-well or 384-well format

Are you interested in seeing the high-throughput Nucleofector® Technology in your lab and how it performs for your cells? One of our transfection specialists can come to your lab, introduce you to your preferred platform and perform a transfection experiment with your cell type of interest.