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Pharma & Biotech

MycoAlertTM PLUS Mycoplasma Detection Kit (30 Tests)

Catalog #: LT07-703
Detect mycoplasma contamination in cell culture supernatants, media, and supplements with the next generation MycoAlertTM Kit allowing the use of less sensitive plate luminometers or multifunctional readers.
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Product Overview

The MycoAlertTM PLUS Assay is a selective biochemical test that exploits the activity of mycoplasmal enzymes which are found in all six of the main mycoplasma cell culture contaminants and the vast majority of 180 mycoplasma species, but are not present in eukaryotic cells. Viable mycoplasma in a test sample (cell supernatant or fresh media or supplements) are lysed and the enzymes react with the MycoAlertTM PLUS Substrate, catalyzing the conversion of ADP to ATP. The ATP is then transferred into a light signal via the luciferase enzyme in the MycoAlertTM PLUS Reagent. By measuring the level of ATP in a sample both before (read A) and after the addition of the MycoAlertTM PLUS Substrate (read B), a ratio can be obtained which is indicative of the presence or absence of mycoplasma.
The MycoAlertTM PLUS Mycoplasma Detection Kit is the next generation MycoAlertTM Kit which provides higher light output and this allows for

  • use with less sensitive plate luminometers or multifunctional readers
  • testing of unused media, media supplements or water

The MycoAlertTM PLUS Mycoplasma Detection Kit is suited for tube luminometers, plate luminometers and multifunctional readers. 

Benefits

  • Easy assay: Simply add two reagents to the culture supernatant and perform two luminescence readings
  • Results in < 20 min
  • Easy interpretation of results

Applications

  • Detects all common mollicute contaminations (Mycoplasma, Acholeplasma, Entomoplasma and Spiroplasma; except Ureaplasma)
  • Suited for tube luminometers, plate luminometers and multifunctional readers

Content & Storage

Content

3 x 1.2 mL MycoAlertTM PLUS Reagent (lyophilized)
3 x 1.2 mL MycoAlertTM PLUS Substrate (lyophilized)
3 x 3 mL MycoAlertTM PLUS Assay Buffer

Instructions

Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

Know Your Enemy – Mycoplasma Contamination in Cell Culture

Webinar

Watch this webinar on mycoplasma testing and learn about potential impacts of a contamination on cell cultures and how you can easily uncover and remove it. 

Get More Info

Interested in Using MycoAlertTM Assays But No Luminometer at Hand?

Check out the LucettaTM 2 Luminometer for performing MycoAlertTM Assays right next to your cell culture hood. Mycoplasma detection couldn't be more convenient.

Request a Trial

Interested in Testing the MycoAlertTM PLUS Assay?

If you are interested in testing the MycoAlertTM Plus Assay, request a trial kit at a 50% discount. The trial kit includes the MycoAlertTM PLUS Assay (10 tests) and the MycoAlertTM Assay Control Set for positive control.

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