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Pharma & Biotech

MycoAlert® PLUS Mycoplasma Detection Kit (50 Tests)

Catalog #: LT07-705

Detection of mycoplasma contamination in cell cultures with less sensitive plate luminometers or multifunctional readers using the MycoAlert® PLUS Mycoplasma Detection Kit (50 Tests).

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Product Overview

Lonza’s MycoAlert® Mycoplasma Detection Assays are selective biochemical tests to detect Mycoplasma contamination in cell cultures. They exploit the activity of mycoplasmal enzymes which are found in the vast majority of about 200 mycoplasma species, but are not present in eukaryotic cells.

Viable mycoplasma in a test sample (cell culture supernatant) are lysed and the released mycoplasmal enzymes react with the MycoAlert® PLUS Substrate, catalyzing the conversion of ADP to ATP. The ATP is then transferred into a light signal via the luciferase enzyme in the MycoAlert® PLUS Reagent. By measuring the level of ATP in a sample both before (read A) and after the addition of the MycoAlert® PLUS Substrate (read B), a ratio can be obtained which is indicative of the presence or absence of mycoplasma.

 

Two versions of MycoAlert® Mycoplasma Detection Assays

Lonza’s MycoAlert® Mycoplasma Detection Assays are available in two versions, the MycoAlert® Mycoplasma Detection Kit and the MycoAlert®PLUS Mycoplasma Detection Kit. Both kits have the same assay principle and the workflow for both assays is the same. However, they do differ in the amount of light each assay emits and therefore have slightly different application ranges. The MycoAlert® Mycoplasma Detection Kit is Lonza’s trusted and long-time favorite Mycoplasma Detection Kit that has been used in more than 7,000 publications. The MycoAlert® PLUS Mycoplasma Detection Kit allows for use with less sensitive plate luminometers or multifunctional readers instead.

 

Positive Control Kit

The positive control for the MycoAlert® PLUS Mycoplasma Detection Kit is available separately in the MycoAlert® Assay Control Set. The control set includes a lyophilized positive control and assay buffer for reconstitution, which also serves as a negative control. The MycoAlert® Assay Control Set is designed for use with the MycoAlert® and MycoAlert® PLUS Mycoplasma Detection Kits. We recommend always using the control set when performing assays. 

Benefits

  • Easy assay: Simply add two reagents to the culture supernatant and perform two luminescence readings
  • Results in < 20 min
  • Easy interpretation of results

Applications

  • Detects all common mollicute contaminations (Mycoplasma, Acholeplasma, Entomoplasma and Spiroplasma; except Ureaplasma)
  • Suited for tube luminometers, plate luminometers and multifunctional readers

Content & Storage

Content

1 x 5 mL MycoAlert® PLUS Reagent (lyophilized)
1 x 5 mL MycoAlert® PLUS Substrate (lyophilized)
1x 10 mL MycoAlert® PLUS Assay Buffer

Instructions

Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

Kinetics of light emission using the MycoAlert™ PLUS Kit

Kinetics of light emission for uninfected and infected cells using the MycoAlert™ PLUS Kit. The B/A ratio indicates the presence or absense of mycoplasma.

Know Your Enemy – Mycoplasma Contamination in Cell Culture

Webinar

Watch this webinar on mycoplasma testing and learn about potential impacts of a contamination on cell cultures and how you can easily uncover and remove it. 

Get More Info

Interested in Using MycoAlert™ Assays But No Luminometer at Hand?

Check out the LucettaTM 2 Luminometer for performing MycoAlertTM Assays right next to your cell culture hood. Mycoplasma detection couldn't be more convenient.

Request a Trial

Interested in Testing the MycoAlert® Assays?

Experience how convenient Mycoplasma testing of your cell culture is using MycoAlert® Assays. Request a trial kit at a 50% discount and test it out!