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Pharma & Biotech

BEGMTM Bronchial Epithelial Cell Growth Medium BulletKitTM

Catalog #: CC-3170

***Limited time offer - Get 30% off with promocode PSTOCKUP on your order. Offer valid in US and select countries in Europe only until March 31, 2019.***

 

Culture system containing BEBMTM Bronchial Epithelial Cell Growth Basal Medium (CC-3171) and BEGMTM Bronchial Epithelial Cell Growth Medium SingleQuotsTM Supplements and Growth Factors (CC-4175).

 

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Product Overview

BEGMTM Bronchial Epithelial Cell Growth Medium is specially designed to support the growth of human primary bronchial epithelial cells. BEGMTM BulletKitTM is serum-free and has been optimized to grow cells from normal and diseased tissues.

Published research shows BEGMTM BulletKitTM has expanded applications. Our customers have used BEGMTM BulletKitTM to culture well-established cell lines such as BEAS-2B, home-grown bronchial epithelial cells and supporting co-culture models with breast cancer cell lines such as MCF-7 and MDA-MB-231. Refer to the published literature in the 'Data' tab.

BEGMTM is an optimized and readily available media kit providing researchers the confidence and the convenience of a system that works to support their cells. This is especially important for researchers who are utilizing cell lines as a stepping stone to primary cell culture. Working with the same BEGMTM Media between cell lines and primary NHBE can reduce variability within the experiments and simplify the shift for researchers. In addition, if primary cells are utilized as a side-by-side control with normal cell lines or home-grown bronchial epithelial cells, the BEGMTMBulletKitTM Media may also offer cost-benefit since customers can use the same media to support multiple cell types.

Here are publications out of many where our customers have used BEGMTM Growth Media with normal bronchial epithelial cells:

  • El Najjar, et al. (2015). PLoSONE 10(2) demonstrates culturing of BEAS-2B bronchial epithelial cell line in BEGMTM Media to examine the effects of cathepsin and furin proteolytic enzymes on viral fusion protein activation in cells
  • Hagiwara, et al. (2015). Nature demonstrates BEGMTM Media can ideally support co-culture of Lonza's HUVEC and NHBE in a 3D environment.
  • Rider, et al. (2015). PLoS ONE 10(1) used BEGMTM Media to culture primary human bronchial epithelial cells obtained from non-transplanted normal lungs

Purchase our Bronchial Epithelial Cell Culture Kit (catalog#. CC-2540B) and get a discounted price on cells and media. The kit consists of a cryopreserved ampule of bronchial epithelial cells (catalog #CC-2540) and BEGMTM BulletKitTM Growth Media (catalog#. CC-3170).

Content & Storage

Content

1 x BEBMTM Basal Medium (CC-3171), 500 mL

1 x BEGMTM SingleQuotsTM Supplement Pack (CC-4175) containing:
1 x Orange Cap Vial with BPE, 2.00 mL
1 x Lilac Cap Vial with Insulin, 0.50 mL
1 x Natural Cap Vial with Hydrocortisone, 0.50 mL
1 x Red Cap Vial with GA-1000, 0.50 mL
1 x Amber Vial with Retinoic Acid, 0.50 mL
1 x Natural Cap Vial with Transferrin, 0.50 mL
1 x Amber Vial with Triiodothyronine, 0.50 mL
1 x Amber Vial with Epinephrine, 0.50 mL
1 x Green Cap Vial with hEGF, 0.50 mL

Instructions

Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Bronchial Epithelial Cells in RAFTTM 3D Cell Culture System

Bronchial Epithelial Cells Grown in BEGMTM BulletKitTM and Cultured in RAFTTM 3D Cell Culture System

Co-culture Experiments with Lonza's Bronchial Epithelial Cells and BEGMTM BulletKitTM Media

Furukawa, et al. demonstrates BEGMTM BulletKitTM is suitable for culturing Lonza's NHBEs as well as to support co-cultures with lung epithelial cell line, BEAS-2B, and breast cancer cell lines, MCF-7 and MDA-MB-231. The breast cancer cell line showed differences in morphology and proliferation rates when cultured with primary NHBE cells vs. BEAS-2B cell line. The paper highlights the importance of using primary NHBE cells as a side-by-side control when conducting experiments with normal lung epithelial cell line such as BEAS-2B because the non-transformed primary cells can validate or offset the data generated using cell lines.

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BEGMTM Bronchial Epithelial Cell Growth Medium

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