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NHBE – Human Bronchial/Tracheal Epithelial Cells with Retinoic Acid

Catalog #: CC-2540

Cryopreserved ampule of Normal Human Bronchial Epithelial (NHBE) cells with Retinoic Acid containing ≥500,000 cells


Interested in reducing lab time? Lonza offers proliferating primary cells in a variety of convenient flask and plate formats. Request more information or quote now.

Do you need a modified version of this cell product, such as different vialing sizes, or isolations from special donors or species? Contact Lonza CellBio Services to discuss custom isolation, expansion, and testing options for your research

 1’967.00 CHF

Product Overview

Normal and Diseased Bronchial Epithelial (NHBE and DHBE) cells are isolated from epithelial lining of airways above bifurcation of the lungs. Lonza's Normal Human Bronchial Epithelial Cells (NHBE) are available with retinoic acid. 

All Cryopreserved human bronchial epithelial cells are guaranteed through 15 population doublings. Cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.

Select Applications with Lonza's Human Bronchial Epithelial Cells -

  • A Rotating Bioreactor for Scalable Culture and Differentiation of Respiratory Epithelium”. Respiratory epithelium is difficult to grow as it requires a well-maintained polarizing air‐liquid interface (ALI) to maintain differentiation. Raredon et al. describe a scalable and easy method to conduct air-liquid interface with Lonza's bronchial epithelial cells guaranteed for ALI differentiation (Cat. No. CC-2540S).
  • Cell concentration and space for growth are required for productive NHBE cell branching in vitro. Click to read this article by Hagiwara et.al that shows how co-cultures with HUVECs in 3D culture influence reconstruction of branched tubular structures in lung epithelial cells.
  • Wu, et al. reports for the first time that HBE cells when grown in B-ALITMMedium can differentiate into 3D glandular acinar structures, likesalivary and mammary epithelial. The cells were grown on the basement membrane matrix Matrigel under select conditions and have shown to exhibit formation of brochospheres or tracheospheres with ciliated lining.

Purchase our Bronchial Epithelial Cell Culture Kit (catalog no. CC-2540B) and get a discounted price on cells and media. The kit consists of a cryopreserved ampule of bronchial epithelial cells (catalog no. CC-2540) and BEGMTMTM BulletKitTM Growth Media (catalog no. CC-3170)


  • All cells test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
  • Certificate of Analysis provided for every cell lot


Content & Storage


1 x Cryopreserved ampule NHBE w/ RA containing ≥500,000 cells


Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

Bronchial Epithelial Cells in RAFT™ 3D Cell Culture System

Bronchial Epithelial Cells Grown in BEGM™ BulletKit™ and Cultured in RAFT™ 3D Cell Culture System

Normal Human Bronchial Epithelial Cell

Normal human bronchial epithelial cell (NHBE) culture showing excellent packed cuboidal morphology

BEGM™ Medium Available

Primary Cell Growth Medium

Our BEGMTM Bronchial Epithelial Cell Growth Medium is fully stocked. We appreciate your business and are dedicated to supporting your research needs.

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