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Pharma & Biotech

NHBE – Human Bronchial/Tracheal Epithelial Cells with Retinoic Acid

Catalog #: CC-2540

Cryopreserved ampule of Normal Human Bronchial Epithelial (NHBE) cells with Retinoic Acid containing ≥500,000 cells

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Product Overview

Normal and Diseased Bronchial Epithelial (NHBE and DHBE) cells are isolated from epithelial lining of airways above bifurcation of the lungs. Lonza's Normal Human Bronchial Epithelial Cells (NHBE) are available with retinoic acid. 

All Cryopreserved human bronchial epithelial cells are guaranteed through 15 population doublings. Cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.

Select Applications with Lonza's Human Bronchial Epithelial Cells -

  • A Rotating Bioreactor for Scalable Culture and Differentiation of Respiratory Epithelium”. Respiratory epithelium is difficult to grow as it requires a well-maintained polarizing air‐liquid interface (ALI) to maintain differentiation. Raredon et al. describe a scalable and easy method to conduct air-liquid interface with Lonza's bronchial epithelial cells guaranteed for ALI differentiation (Cat. No. CC-2540S).
  • Cell concentration and space for growth are required for productive NHBE cell branching in vitro. Click to read this article by Hagiwara et.al that shows how co-cultures with HUVECs in 3D culture influence reconstruction of branched tubular structures in lung epithelial cells.
  • Wu, et al. reports for the first time that HBE cells when grown in B-ALITMMedium can differentiate into 3D glandular acinar structures, likesalivary and mammary epithelial. The cells were grown on the basement membrane matrix Matrigel under select conditions and have shown to exhibit formation of brochospheres or tracheospheres with ciliated lining.

Purchase our Bronchial Epithelial Cell Culture Kit (catalog no. CC-2540B) and get a discounted price on cells and media. The kit consists of a cryopreserved ampule of bronchial epithelial cells (catalog no. CC-2540) and BEGMTMTM BulletKitTM Growth Media (catalog no. CC-3170)

Benefits

  • All cells test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
  • Certificate of Analysis provided for every cell lot

 

Content & Storage

Content

1 x Cryopreserved ampule NHBE w/ RA containing ≥500,000 cells

Instructions

Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

Bronchial Epithelial Cells in RAFTTM 3D Cell Culture System

Bronchial Epithelial Cells Grown in BEGMTM BulletKitTM and Cultured in RAFTTM 3D Cell Culture System

Normal Human Bronchial Epithelial Cell

Normal human bronchial epithelial cell (NHBE) culture showing excellent packed cuboidal morphology

BEGMTM Medium Available

Primary Cell Growth Medium

Our BEGMTM Bronchial Epithelial Cell Growth Medium is fully stocked. We appreciate your business and are dedicated to supporting your research needs.

CytoSMARTTM 2 System

Special Sale: Up to 64% Off*

Save up to 64% when ordering a CytoSMARTTM 2 System and equip your lab now with our easy-to-use live cell monitoring device.

 

*Order by 30 November 2019 to take advantage of this special offer. Available in participating countries only.

Need a Quote or Any Support?

Are Lonza's primary cells and media applicable to your project? Do you need a quote or any help? Contact us and a local representative will get back to you.

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