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CompareP3 Primary Cell 384-well Nucleofector® KitCatalog #: V5SP-3010For high-throughput transfection of primary cells, e.g. human T cells, DCs or CD34, when working with the 384-well Nucleofector® System.Sales unit: items
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CompareSF Cell Line 384-well Nucleofector® KitCatalog #: V5SC-2010
For high-throughput transfection of cell lines, e.g. K-562, Neuro-2A or RAW246.7, when working with the 384-well HT Nucleofector® System.
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CompareSF Cell Line 384-well Nucleofector® KitCatalog #: V5SC-2002
For high-throughput transfection of cell lines, e.g. K-562, Neuro-2A or RAW246.7, when working with the 384-well HT Nucleofector® System.
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CompareInspection Equipment Nucleofector® II/2b SystemCatalog #: AXP-1001For testing the functionality of your Nucleofector® 2b SystemSales unit: items
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AccuGENE® 10X Tris-Glycine SDS Buffer, 4L
Catalog #: 50882AccuGENE® 10X Tris-Glycine Buffer, 4 L, for SDS-PAGE
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CompareNHEK-Ad - Human Epidermal Keratinocytes, Adult, Single DonorCatalog #: 00192627
Cryopreserved Human Adult Epidermal Keratinocytes (NHEK-Ad) from a single donor, ≥ 500,000 cells
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ComparePyroCell® Monocyte Activation Test – Human Serum KitCatalog #: 00279770
The PyroCell® Monocyte Activation Test – Human Serum Kit combines 3 vials of cryopreserved, pooled PBMC (human, pMAT cells), and 3 vials of cryopreserved Human Serum Supplement, for 288 tests (3x 96-well microtiter plates).
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ComparePyroCell® Monocyte Activation Test (MAT) KitCatalog #: 00249735
PyroCell® Monocyte Activation Test (MAT) Kit qualified for the monocyte activation test including 3 vials pooled PBMC (pMAT cells, 4 donors) as well as 3 vials of Cell Culture Medium Supplement for 3 x 96-well ELISA plates.
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CompareFlashGel® Recovery KitCatalog #: 57064
FlashGel® Recovery Kit, including a box of 1.2% FlashGel® DNA Cassettes, FlashGel® Loading Dye (5X), FlashGel® Recovery Buffer, FlashGel® DNA QuantLadder, FlashGel® Visualization Glasses, and FlashGel® Mask
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CompareLatitude® HT Precast Gel TBECatalog #: 57246
Latitude® HT (24 × 14 cm) 2%, 4 × 25-well, 5 gels
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Hematopoietic and Immune Cell Products - Essential Tools for Hematopoietic ResearchLonza_Brochures_Hematopoietic_and_Immune_Cell_Products_-_Essential_Tools_for_Hematopoietic_Research.pdf
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References - X-Vivo™Overview of relevant references
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Live-cell imaging of CD34+ cells with Nanolive’s 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, in the presence of recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell migration was captured. -
Mitosis of CD34+ cells with Nanolive's 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, supplemented with recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell division was captured. One can distinguish characteristic steps of mitosis affirming that Nanolive’s technology was completely non-invasive. -
Terms and ConditionsCD34+ cells BOGO
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Human CD34+ Cells Technical information and instructions for use
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Instructions - Neural Progenitor Cell System, NHNPInstructions for Proper Use
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TechSheet - Neural Progenitor Cell System, NHNPTechnical Product Information Sheet
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Transfection Protocol for Human CD34+ Cells - HT Nucleofector™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell HT-Nucleofector™ Kit
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Transfection Protocol for Human CD34+ Cells - 96-well Shuttle™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell 96-well Nucleofector™ Kit