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NHEK-Ad - Human Epidermal Keratinocytes, Adult, Single Donor

NHEK-Ad - Human Epidermal Keratinocytes, Adult, Single Donor

Catalog #: 00192627

Cryopreserved Human Adult Epidermal Keratinocytes (NHEK-Ad) from a single donor, ≥ 500,000 cells


Interested in reducing lab time? Lonza offers proliferating primary cells in a variety of convenient flask and plate formats. Request more information or quote now.

Do you need a modified version of this cell product, such as different vialing sizes, or isolations from special donors or species? Contact Lonza CellBio Services to discuss custom isolation, expansion, and testing options for your research


 900.00 CHF

Product Overview

Cryopreserved Normal Human Epidermal Keratinocytes are guaranteed through 18 population doublings for adult cells. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased. Normal Human Dermal fibroblasts from matched donors are available for certain lots.

Purchase our Adult Keratinocyte Cell Culture Kit (catalog no. 00192627B) and get a discounted price on cells and media. The kit consists of a cryopreserved ampule of adult keratinocytes (catalog no. 00192627) and KGMTM-Gold BulletKitTM Growth Media (catalog no. 00192060)

References with Lonza’s Keratinocytes:

  • Protective barrier: Keratinocytes were stimulated with histamine or histamine receptor ligands to show the formation of a defective skin barrier. Gschwandtner M, Mildner M,  Mlitz V,  Gruber F,  Eckhart L, Werfel T, Gutzmer R, Elias PM and Tschachler E.  Histamine suppresses epidermal keratinocyte differentiation and impairs skin barrier function in a human skin model. European Journal of Allergy and Clinical immunology (2013); 68(1): 37–47. 
  • Skin model: Neonatal keratinocytes were cultured in a microfluidic system to precisely examine the morphology, behavior and viability of individual cells. Adrian T. O’Neill, Nancy A. Monteiro-Riviere, and Glenn M. Walker. Characterization of microfluidic human epidermal keratinocyte culture. Cytotechnology (2008); 56(3): 197–207. 
  • Cancer: Keratinocytes were used in this study to demonstrate that dysregulated activation of type I interferon innate immunity is implicated in the molecular processes triggered by anti-EGFR drugs and leading to persistent skin inflammation. Carrie HS, Hong Y, Yunbo L, Jennifer SM, Sridevi B, Gaylene S, Judith AC and Thomas RS.  EGF receptor signaling blocks aryl hydrocarbon receptor-mediated transcription and cell differentiation in human epidermal keratinocytes. Proceedings of National Academy of Sciences (2009); 106(11): 4266-71. 
  • Human Papillomaviruses (HPV) mediated skin cancer response: An infection of the skin with human papillomaviruses (HPV) may result in benign tumors with limited growth, which tend to regress spontaneously and causes non-melanoma skin cancer development in epidermodysplasia verruciformis (EV) and immunosuppressed patients.  The present study utilized pooled keratinocytes cultured in KGMTM-Gold Medium from several healthy donors. The data showed that betaPV may increase the number of stem cell-like cells present during early carcinogenesis and thus enable the persistence and accumulation of DNA damage necessary to generate malignant stem cells. Martin H, Adrian B, Cinzia B, Marisa G, John D, Alan S, Herbert P, Ian M and Baki A. Expression of betapapillomavirus oncogenes increases the number of keratinocytes with stem cell-like properties. Journal of Virology (2013); 87(22): 12158-12165. 

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Storage and Content

  • 1 x Cryopreserved ampule of NHEK-Ad from single donor containing ≥ 500,000 cells

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SDS, CoA, and Instructions

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

  • TechSheet - Keratinocyte Cell Systems

    Technical information sheet for Clonetics™ for Keratinocyte Cell Systems
  • TechSheet - Keratinocyte Cell Medium Products

    Technical information sheet for Clonetics™ Keratinocyte Media
  • Instructions - Keratinocyte Cell Systems

    Clonetics™ Human Epidermal Keratinocyte Cell Systems - Instructions For Use
  • Co-culturing Lonza Matched Donor Keratinocytes and Fibroblasts

    A High-throughput, 2D Skin Model
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Educational Material

Brochures, White Papers etc.

  • White Paper

    Live Cell Monitoring with the CytoSMART™ System: Application Examples
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Normal Human Epidermal Keratinocytes

Cryopreserved Normal Human Epidermal Keratinocytes are guaranteed through 18 population doublings for adult cells and 20 population doublings for neonatal cells

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