NHDF-Ad - Human Dermal Fibroblasts, Adult
NHDF-Ad - Human Dermal Fibroblasts, Adult
Cryopreserved ampule of Normal Adult Human Dermal Fibroblasts (NHDF-Ad) containing ≥ 500,000 cells
Interested in reducing lab time? Lonza offers proliferating primary cells in a variety of convenient flask and plate formats. Request more information or quote now.
Do you need a modified version of this cell product, such as different vialing sizes, or isolations from special donors or species? Contact Lonza CellBio Services to discuss custom isolation, expansion, and testing options for your research
Product Overview
Human Dermal Fibroblasts are available from either adult skin (NHDF-Ad) or neonatal foreskin (NHDF-Neo). They are derived from the dermis of normal human neonatal foreskin or adult skin. They are cryopreserved at the end of primary culture. Normal Human Epidermal Keratinocytes (00192627) from matched donors are available for certain lots
Lonza's human dermal fibroblasts are guaranteed through 15 population doublings when using FGMTM-2 Growth Media. Dermal fibroblasts are characterized by morphological observation throughout serial passage.
All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.
Request More InfoApplications
- ECM protein analysis
- Wound healing
- Collagen metabolism
- Cosmetics
- Skin therapy/models
Storage and Content
1 x Cryopreserved ampule of NHDF-Ad containing ≥ 500,000 cells
SDS, CoA, and Instructions
Safety Data Sheets (SDS)
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Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
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Primary Cells and Media Reference Guide
A comprehensive collection of Lonza primary cell and media products supporting many research areas. -
Instructions - Human Fibroblast Cell Systems
Technical Instructions for culturing Clonetics™ Human Fibroblasts Cells -
Co-culturing Lonza Matched Donor Keratinocytes and Fibroblasts
A High-throughput, 2D Skin Model -
Human Umbilical Vein Endothelial Cell and Normal Human Dermal Fibroblast Co-Culture and Angiogenesis Assay
Instructions for Use -
A Quantitative, High-throughput, Real-time Angiogenic Tube Formation Assay
Angiogenic Assay - Human Umbilical Vein Endothelial Cells and Human Dermal Fibroblasts in Co-culture -
Instructions for use – Matched donor Keratinocytes and Fibroblasts 2D Co-Culture Protocol
Details provided for how to establish a Keratinocyte and Dermal Fibroblast co-culture
Educational Material
Brochures, White Papers etc.
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Primary Cells and Media Reference Guide
A comprehensive collection of Lonza primary cell and media products supporting many research areas. -
Primary Cell Types Provide a Ready-to-Use Platform for Cell-Based Assays
Poster presented at Miptec, Basel (CH) - Sep 21-24, 2010 -
A Serum-free Chemically Defined Medium for the Expansion of NHDF-Adult
Traditionally, in vitro cell culture medium contains animal-derived products like serum, tissue extracts or hydrosylates. They are also a source of undesirable factors, such as endotoxin, and pose potential BSE risks. The undefined and variable nature of these components is a challenge in basic research and applied biomedical sciences. Lonza has developed a serum-free chemically defined, fibroblast growth medium, to propagate primary adult normal human dermal fibroblasts (NHDF). -
A Quantitative, High-throughput, Real-time Angiogenic Tube Formation Assay
Angiogenic Assay - Human Umbilical Vein Endothelial Cells and Human Dermal Fibroblasts in Co-culture
NHDF-Ad - Human Dermal Fibroblasts, Adult
Immunohistochemistry staining of normal human adult dermal fibroblasts (CC-2511) epanded into a 24 well plate. Cells were stained with primary antibody Anti-Vimentin AB24525 (AbCam) and secondary antibody Alexa 594, Goat anti Chicken, A11042 Invitrogen was used at 1:200 dilution.
