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HMVEC-dAd – Human Dermal Microvascular Endothelial Cells – Adult

HMVEC-dAd – Human Dermal Microvascular Endothelial Cells – Adult

Catalog #: CC-2543

Cryopreserved ampule of Adult Human Dermal Microvascular Endothelial Cells (HMVEC-d Ad) containing ≥ 500,000 cells

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 1’696.00 CHF
EA

Product Overview

Human Dermal Microvascular Endothelial Cells include cells isolated from small vessels within skin tissue from either adults or neonatal foreskins.  Cells are cultured to be ≥90% pure and specifically enriched for dermal LECs and BECs. Dermal microvascular endothelial cells, from either neonates or adults (HMVEC-D) are a mixed population of both blood (HMVEC-DBl) and lymphatic (HMVEC-DLy) endothelial cells.                                      

Adult cells are designated with an 'Ad'; neonate cells are designated with 'Neo'.  Both pooled and single donors are available. Additional donor characteristics may be available by contacting our Scientific Support Team. Endothelial Cells and Media are convenient and easy to use, allowing the researcher to focus on results.  Lonza's Cells, Medium and Reagents are quality tested together and guaranteed to give optimum performance as a complete cell system.  

Cryopreserved Adult Human Dermal Microvascular Endothelial Cells are shipped in third passage. Cryopreserved Dermal Microvascular Endothelial Cells are guaranteed through 15 population doublings (CC-2543, CC-2505 & CC-2516) and 12 population doublings (CC-2810, CC-2811, CC-2812 & CC-2813) , express CD31/105, von Williebrand Factor VIII, and are positive for acetyated low density lipoprotein uptake.  All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.

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Benefits

  • Ready-To-Use 
  • No tissue dissection required 
  • Increased data reproducibility
  • Characteristic morphology and staining tests confirm tissue origin and cell type
  • Negative for mycoplasma, bacteria, yeast and fungi
  • Certificate of Analysis (CoA) is available for every batch 
 

 

Storage and Content

  • 1 x Cryopreserved Ampule with ≥500,000 cells/vial
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SDS, CoA, and Instructions

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

  • Primary Cells and Media Reference Guide

    A comprehensive collection of Lonza primary cell and media products supporting many research areas.
  • Instructions & Technical Info - Endothelial Cell Systems

    Technical Information and Instructions for Proper Use of Endothelial Cells
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Educational Material

Brochures, White Papers etc.

  • Primary Cells and Media Reference Guide

    A comprehensive collection of Lonza primary cell and media products supporting many research areas.
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Data

HMVEC-dAd – Human Dermal Microvascular Endothelial Cells – Adult

Human microvascular endothelial cells (CC-2543) were stained for von Willebrand Factor using antibody (Sigma, F3520) at 1:200 dilution (green). Secondary antibody used was VECTDI1088 DyLight 488 (Vector Laboratories). To detect CD31/PECAM-1, cells were stained with (R&D Systems, BBA7) at 1:100 dilution(red). Secondary antibody used was VECTDI2594 DyLight 594 (Vector Laboratories).

Data

HMVEC-dAd – Human Dermal Microvascular Endothelial Cells – Adult

Human microvascular endothelial cells (CC-2543) were stained for von Willebrand Factor using antibody (Sigma, F3520) at 1:200 dilution (green). Secondary antibody used was VECTDI1088 DyLight 488 (Vector Laboratories). To detect CD31/PECAM-1, cells were stained with (R&D Systems, BBA7) at 1:100 dilution(red). Secondary antibody used was VECTDI2594 DyLight 594 (Vector Laboratories).

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EGM™-2 Medium Available

Primary Cell Growth Medium

Our EGMTM-2 Endothelial Cell Growth Medium is fully stocked. We appreciate your business and are dedicated to supporting your research needs.

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