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Pharma & Biotech

KGMTM Gold Keratinocyte Growth Medium BulletKitTM

Catalog #: 00192060

***Limited time offer - Get 30% off with promocode PSTOCKUP on your order. Offer valid in US and select countries in Europe only until March 31, 2019.**

 

Culture system containing KBMTM GoldTM Basal Medium (00192151) and KGMTM GoldTM SingleQuotsTMsupplements (00192152) required for growth of human keratinocytes.

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Product Overview

A new robust and versatile serum-free medium formulation for high and low density NHEK-Normal Human Epidermal Keratinocyte cultures

KGM-GoldTM Keratinocyte Growth Medium is designed to support both clonal growth and high density keratinocyte proliferation. The medium supports superior growth and morphology of keratinocytes isolated in KGM-2&trade, KGM&trade and Competitor A's Medium (data shown in the data tab)

Furthermore, quality control at Lonza requires that keratinocytes pass strict morphological criteria specifying the minimal presence of enlarged and vacuolated cells, enabling Lonza keratinocytes to be used by the researcher for ≥15 Population Doublings. 

Purchase our Adult Keratinocyte Cell Culture Kit (catalog no. 192627B) and get a discounted price on cells and media. The kit consists of a cryopreserved ampule of adult keratinocytes (catalog no. 192627) and KGMTM-Gold BulletKitTM Growth Media (catalog no. 192060)

Content & Storage

Content

1 x KBMTM GoldTM Basal Medium (00192151), 500 mL

1 x KGMTMGoldTM SingleQuotsTM Supplement pack (00192152) containing: 
1 x Natural Cap Vial with Hydrocortisone, 0.50 mL
1 x Natural Cap Vial with Transferrin, 0.50 mL
1 x Amber Vial with Epinephrine, 0.25 mL
1 x Red Cap Vial with GA-1000, 0.50 mL
1 x Orange Cap Vial with BPE, 2.00 mL
1 x Green Cap Vial with hEGF, 0.50 mL
1 x Lilac Cap Vial with Insulin, 0.50 mL

Instructions

Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Morphology of Lonza’s Adult Keratinocytes Grown in KGMTM Gold Growth Medium Compared to Competitor A’s Adult Keratinocytes in Their Medium.

The growth performance and longevity of Competitor A's adult keratinocytes grown in Competitor A's Medium were compared with Lonza’s adult keratinocytes grown in KGM-GoldTM Growth Medium. Although Competitor A claims ≥25 total population doublings for their adult keratinocytes, actual results show that this lot barely obtained 19 PD. Note the presence of several enlarged cells in Passage 2 in Competitor’s Medium. At the end of passage 4 (15 PD) Lonza’s adult keratinocytes consist of mostly small proliferative cells.

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