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CompareHuman Melanocyte - Neonatal Nucleofector® KitCatalog #: VPD-1003For efficient transfection of primary human melanocytes in the Nucleofector® 2b DeviceSales unit: items
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CompareHuman B Cell Nucleofector® KitCatalog #: VPA-1001
For efficient transfection of primary human B cells in the Nucleofector® I/II/2b Device
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CompareMouse/Rat Hepatocyte Nucleofector® KitCatalog #: VPL-1004
For efficient transfection of primary mouse or rat hepatocytes in the Nucleofector® I/II/2b Device
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ComparePrimary Cell Optimization 384-well Nucleofector® KitCatalog #: V5SP-9001For high-throughput optimization of transfection conditions for primary cell types lacking an Optimized Protocol for the 384-well HT Nucleofector® SystemSales unit: items
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CompareHuman Natural Killer Cell Nucleofector® KitCatalog #: VPA-1005
For efficient transfection of primary NK (natural killer) cells in the Nucleofector® I/II/2b System
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CompareMouse Embryonic Stem Cell Nucleofector® KitCatalog #: VPH-1001
For efficient transfection of primary mouse embryonic stem cells in the Nucleofector® I/II/2b System
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CompareHuman Mesenchymal Stem Cell Nucleofector® KitCatalog #: VPE-1001
For efficient transfection of primary human mesenchymal stem cells in the Nucleofector® I/II/2b System
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CompareMouse Neural Stem Cell Nucleofector® KitCatalog #: VPG-1004
For efficient transfection of primary mouse neural stem cells in the Nucleofector® I/II/2b Device
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CompareHuman Mammary Epithelial Cell Nucleofector® KitCatalog #: VPK-1002
For efficient transfection of primary human mammary epithelial cells in the Nucleofector® I/II/2b Device
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CompareHuman Umbilical Vein Endothelial Cell Nucleofector® KitCatalog #: VPB-1002
For efficient transfection of primary HUVECs in the Nucleofector® 2b System
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TechSheet - Umbilical Vein Endothelial Cell SystemsTechnical information sheet for CloneticsTM HUVEC cells
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Instructions for Use - HUVEC Culturing and Angiogenesis Inhibition Assay using Sartorius Incucyte®Protocol for Angiogenesis assay using HUVEC cells
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Tube Formation Assay with Primary HUVECsIn this study, we have optimized this assay using Lonza's Primary HUVECs and EGM®-2 Media
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Expanded HUVECs for High-throughput ScreeningHUVECs are a good primary endothelial cell type for many in vitro studies and also for several high-throughput screening applications. Consequently, Lonza offers, HUVEC-XL. These cells are expanded to passage-3 from pooled P1-HUVECs and packaged at 10 million cells per ampoule.
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Tube Formation Assay with HUVECHuman Umbilical Vein Endothelial Cells seeded on BME and the formation of endothelial tubes were monitored live with the CytoSMART™ Lux 10X System, the previous version of the CytoSMART™ 2 System. Time-lapse video was recorded for 22 hours at a frame interval of 1 frame every 5 minutes. -
HUVEC-Fibroblast Co-Culture - AngiogenesisTime-Lapse Video of Human Umbilical Vein Endothelial Cell Angiogenic Tube Formation in Co-Culture with Dermal Fibroblasts. Primary Human Umbilical Vein Endothelial Cells (HUVECs) were co-cultured with primary Human Normal Dermal Fibroblast cells at a ratio of 1:3 HUVECs: fibroblasts and a total cell density of 40,000 cells/well in a 96-well plate. Co-cultures were maintained for 5 days with medium changes every 2-3 days in EBMTM-2 medium supplemented with a custom concentration of VEGF (313 pM) to optimize angiogenic tube formation in this setting. Cells were stained with CellTracker® Dye from ThermoFisher. Purple lines indicate angiogenic tube formation detected by the live cell imaging software. At each frame, the software quantifies angiogenesis by counting the number of network branch points (i.e., the number of branches in each network of tubes) per mm2 and the mean network length (i.e., the average of the total network length of tubes per mm2). -
HUVEC-Fibroblast Co-Culture – Angiogenesis Inhibition -
Automated Cell Signaling Assays Using Primary HUVECsLonza's Primary HUVECs are an excellent cell type to measure the effects of pharmacologically-important compounds on vial signaling pathways, and they provide increased data relevancy compared to cell lines.
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Lonza Extends Industry-Standard HUVEC Product LineLonza, the market leader in primary cells, has expanded its human primary cell portfolio with an improved version of Human Umbilical Vein Endothelial Cells (HUVEC) cultured in the absence of additive vascular endothelial growth factor (VEGF).
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Instructions - HUVEC-XL™ Pooled Cell SystemInstructions for culturing Clonetics™ HIVEC-XL™Pooled Cells
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