Primary Cell Optimization 384-well Nucleofector™ Kit
What You May Also Need
Product Overview
The Primary Cell Optimization 384-well NucleofectorTM Kit is suited for high-throughput optimization of transfection conditions for primary cell types lacking an Optimized Protocol for the 384-well HT NucleofectorTM System.
Complete Optimization: Different conditions can easily be tested within one experiment using a 384-well NucleocuvetteTM Plate and our pmaxGFPTM Vector as positive control. Our five Primary Cell NucleofectorTM Solutions P1 – P5 are tested in combination with a pre-selected set of programs plus controls. A subsequent optional fine tuning of the Nucleofection conditions can be performed with the help of our scientific support team.
Cell Group-Specific Optimization using Basic Protocols: In addition to a complete optimization described above, we also provide a cell group-specific optimization protocol for some primary cell groups. Those so called Basic Protocols refer to a pre-selected set of 1 or 2 Primary Cell NucleofectorTM Solutions and NucleofectorTM Programs. Basic Protocols are available for following cell groups:
- Endothelial cells (mammalian)
- Epithelial cells (mammalian)
- Fibroblasts (mammalian)
- Glial cells (mammalian)
- Neurons (mammalian)
- Smooth muscle cells (mammalian)
- Stem cells (human)
Before doing an optimization for your cell type of interest, please check out our knowledge database for availability of a ready-to-use Lonza Optimized Protocol or any existing data.
Benefits
- High transfection efficiencies for primary cells
- Automated high-throughput electroporation
- Convenient and rapid determination of optimal Nucleofection conditions for a broad range of primary cells within one experiment
- Optimal Nucleofection conditions determined on the 384-well HT NucleofectorTM System are transferable to the 4D-NucleofectorTM System or the 96-well ShuttleTM Add-On
Applications
- Transfection of low cell numbers in the range of 2 x 104 to 1 x 106 cells
- Determination of Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 384-well HT NucleofectorTM System
Content & Storage
Content
2.25 mL NucleofectorTM Solution P1
2.25 mL NucleofectorTM Solution P2
2.25 mL NucleofectorTM Solution P3
2.25 mL NucleofectorTM Solution P4
2.25 mL NucleofectorTM Solution P5
4 x 0.15 mL Supplement 1
1 x 0.15 mL Supplement 3
150 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
1 x 384-well NucleocuvetteTM Plate
