Cell Line Optimization 384-well Nucleofector™ Kit
What You May Also Need
Product Overview
The Cell Line Optimization 384-well NucleofectorTM Kit is suited for high-throughput optimization of transfection conditions for primary cell types lacking an Optimized Protocol for the 384-well HT NucleofectorTM System.
Different conditions can easily be tested within one experiment using a 384-well NucleocuvetteTM Plate and our pmaxGFPTM Vector as positive control. Our three Cell Line NucleofectorTM Solutions SE, SF and SG are tested in combination with a pre-selected set of programs plus controls. A subsequent optional fine tuning of the Nucleofection conditions can be performed with the help of our Scientific Support Team.
Before doing an optimization for your cell type of interest, please check out our knowledge database for availability of a ready-to-use Lonza Optimized Protocol or any existing data.
Benefits
- High transfection efficiencies for cell lines
- Automated high-throughput electroporation
- Convenient and rapid determination of optimal Nucleofection conditions for a broad range of cell lines within one experiment
- Optimal Nucleofection conditions determined on the 384-well HT NucleofectorTM System are transferable to the 4D-NucleofectorTM System or the 96-well ShuttleTM Add-On
Applications
- Transfection of low cell numbers in the range of 2 x 104 to 1 x 106 cells
- Determination of Nucleofection conditions for cell lines lacking an Optimized Protocol for the 384-well HT NucleofectorTM System
Content & Storage
Content
2.25 mL NucleofectorTM Solution SE
2.25 mL NucleofectorTM Solution SF
2.25 mL NucleofectorTM Solution SG
3 x 0.15 mL Supplement 1
150 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
1 x 384-well NucleocuvetteTM Plate
