A white paper exploring differential gene expression in primary human lung fibroblasts

Access to diseased primary cells from patients diagnosed with COPD or asthma provides a convenient, biologically relevant model to assess genetic and in some cases phenotypic changes from normal, healthy donor tissues. In this study, we grew human bronchial epithelial (HBE) cells from normal, COPD-, and asthma-diagnosed human donor lung tissues in an air-liquid interface (ALI) model to assess potential phenotypic changes in vitro as well as provide a differentiated layer of cells to harvest for gene expression studies using the StellARray™ Gene Expression System.

Our data demonstrate that SAEC can be differentiated in an ALI culture system that is capable of forming tight junctions, displaying cilia and secreting ASL. This system should facilitate more physiologically relevant studies of both normal SAEC and those from diseases, such as COPD and asthma.

Lonza is pleased to announce that the Clonetics™ Primary Cells and Media product line is celebrating its 30th anniversary, supporting cell culture research.

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Protocol for cold trypsinisation (for serum free cell cultures)

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Technical information and instructions for culturing CloneticsTM Conditionally Immortalized Human Brain Microvascular Endothelial Cell

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Software Manual Nucleofector™ 96-well Shuttle™ System (Version 1.2b)