SF Cell Line 4D-Nucleofector® X Kit L
SF Cell Line 4D-Nucleofector® X Kit L
For efficient transfection of difficult-to-transfect cell lines, e.g. K-562, Neuro-2A or RAW264.7, in the 4D-Nucleofector® X Unit (100 µL format).
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Product Overview
The SF Cell Line 4D-Nucleofector® X Kit L is one of our three 4D-Nucleofector® Kits that is suited for transfecting difficult-to-transfect cell lines in the 100 µL Nucleocuvette® Vessel format when working with the 4D-Nucleofector® X Unit.
This kit has been determined to be optimal for transfecting following cell types:
- 293-F (FreeStyle), 32D
- A-20, A2058, A375, A431, A549, AGS, ARPE-19
- BetaTC/6, BxPC-3
- C6, CCRF-CEM, CHO-K1, CHO-S (Invitrogen), Cos-1
- EL4
- FDC-P1
- H9c2 (2-1), HEK293, Hel9217, HepG2, High 5, HL-60, HMC-1-8, HT-1080, HT-29, Hut-78, HUV-EC-C
- IMR-32
- J774A.1
- K-562
- KG-1
- L-428, LNCaP
- MDA-MB-453, MDBK, Molt-4, MV-4-11
- Nalm-6, NB-4, NCI-H1299 [H1299], Neuro-2a [N2a], NG108-15, NS0
- P19, P815, PC-12, PC-3
- Raw 264.7, RBL-1, RBL-2H3, Reh, Rin m5F, RPMI 8226
- S2, S49, SaOS, SF9, SH-SY5Y, SK-BR-3, SK-N-AS, SK-N-MC, SK-N-SH, SK-OV-3, Sp2/0-Ag14, SW480
- T2, T84, TF-1
- U266B1, U-937
- Vero
The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
The kit is available in different variants for either 12 or 24 reactions.
Request More InfoBenefits
- High transfection efficiencies in difficult-to-transfect cell lines
- Cells maintain functionality post transfection
- Conditions are transferable between 4D-Nucleofector® Systems (including the 96-well Shuttle® System and 384-well Nucleofector® System
Applications
- Transfection of cell numbers in the range of 2 x 105 to 2 x 107 cells
- Low throughput applications
Storage and Content
2.25 mL SF Cell Line Nucleofector® Solution
0.5 mL Supplement 1
50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
24 x 100 µL Nucleocuvette® Vessel
24 x Single use pipettes
SDS, CoA, and Instructions
Safety Data Sheets (SDS)
Choose a language to view the SDS.
Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
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4D-Nucleofector® Protocol for TF-1
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for TF-1 -
4D-Nucleofector® Protocol for A-375
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for A-375 -
4D-Nucleofector® Protocol for CCRF-CEM
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for CCRF-CEM -
4D-Nucleofector® Protocol for C6 Glioma
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for C6 Glioma -
4D-Nucleofector® Protocol for L428
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for L428 -
4D-Nucleofector® Protocol for FDC-P1
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for FDC-P1 -
4D-Nucleofector® Protocol for CHO-K1
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for CHO-K1 -
4D-Nucleofector® Protocol for EL4
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for EL4 -
4D-Nucleofector® Protocol for K562
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for K562 -
4D-Nucleofector® Protocol for HL-60
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for HL-60
