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4D-Nucleofector® Core Unit

Catalog #: AAF-1003B

The controlling unit of the 4D-Nucleofector® System is suited to handle various functional units for efficient transfection of hard-to-transfect cell lines and primary cells in different formats.


Product Overview

The 4D-Nucleofector® Core Unit is the controlling unit of the 4D-Nucleofector® System. The different functional units can be added in a modular way to the 4D-Nucleofector® Core Unit and each is suited for different applications:

  • X Unit: Supporting Nucleofection® of various cell numbers in different formats (16-well Nucleocuvette® Strips or 100 µL Nucleocuvette® Vessels
  • Y Unit: Enabling adherent Nucleofection® in 24-well culture plates
  • LV Unit: Large-scale transfection of up to 1x109 cells
  • 96-well Unit: Higher throughput Nucleofection® Experiments in 96-well Nucleocuvette® Plates

Transfections per minute:

  • X Unit (16-well Nucleocuvette® Strips): 16-32 reactions
  • X Unit (100 µL Nucleocuvette® Vessels): 20-26 reactions
  • Y Unit (Dipping Electrodes): 8-10 reactions
  • LV Unit (LV Nucleocuvette® Cartridges): 2-3 mL
  • 96-well Unit (96-well Nucleocuvette® Plate): 32 - 44 reactions


There are cell type-specific Optimized Protocols or recommendations available in our knowledge database. In these Optimized Protocols the best Nucleofection® Conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.


Technical Specifications

Dimensions: (w x h x d): 24.5×14.7×28 cm / 9.7×5.8×11.0

Weight: 4.4 kg ( 9.7 lb)

Power: 100 - 240 VAC

Frequency: 50 - 60 Hz

Consumption: 0 VA (power off) / 20 VA (idle) / 140 VA (busy - during transfection)

Fuse: T 2.5A, L250V

Operating temperature: +15°C up to +40°C (59°F - 104°F)

Environment non condensing

Voltage limitation: 1500 V

Current limitation: 100 A



The Core Unit can be used to operate the following functional units:

  • X Unit
  • Y Unit
  • LV Unit 
  • 96-well Unit

This Core Unit is not compatible with the 96-well ShuttleTM Add-on



  • Controls attached functional units
  • 8’’ touch screen to operate the system
  • Intuitive operation software for seamless designing and saving individual experimental setups
  • Includes USB and network connectivity for software update and data transfer


Controlling the 4D-Nucleofector® System

Content & Storage


1 Unit


Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

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