4D-Nucleofector® 96-well Unit
4D-Nucleofector® 96-well Unit
The medium-throughput module for the 4D-Nucleofector® Platform suited for transfection of hard-to-transfect cell lines or primary cells in 96-well format
Product Overview
The 4D-Nucleofector® 96-well Unit is one of the four functional modules of the 4D-Nucleofector® System. The 96-well Unit is a medium-throughput module for the 4D-Nucleofector® System suited for convenient optimization of Nucleofection conditions or as an assay establishment tool.
For integration of the 96-well Unit into a liquid handling workstation we offer a separate Automation Server Software (SBA-3001).
There are cell type-specific Optimized Protocols or recommendations available in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
Technical Specifications
Dimensions: (w × d × h) 24.5 × 10.5 × 28 cm / 9.7 × 4.1 × 11.0 in
Weight: 4.7 kg (10.4 lb)
Power supply: 110 VAC +10%/-20% or 240 VAC +10%/-20% 50–60 Hz, self–regulating
Power consumption: 0 VA (power off) / 20 VA (idle) / 140 VA (busy - during transfection)
Compatibility
The new 96-well Unit is only compatible with the 4D-Nucleofector® Core Unit with part code AAF-1003B
With the introduction of the Next Generation 4D-Nucleofector® System, the 96-well Shuttle® Add-on is no longer available. It is replaced by the new 4D-Nucleofector® 96-well Unit as a fully integrated module for the Next Generation 4D-Nucleofector® Platform.
Please note: The 96-well Shuttle® Add-on cannot be operated with the Next Generation 4D-Nucleofector® Core Unit and vice versa. The new 96-well Unit cannot be integrated in the 4D-Nucleofector® System of the first generation (Core Unit part code AAF-1001B, or AAF-1002B).
Benefits
- Up to 96 independent programs can be run per plate, processed automatically in <2 minutes
- Modular 6 × 16-well Nucleocuvette® Plate for scalable throughput
- Fulfills pre-requisites for liquid handling integration
Applications
- Convenient optimization of any difficult-to-transfect cell line in just 1 plate
- Variable cell numbers from 104 – 106 cells per reaction
- Testing of multiple conditions for assay establishment
- Screening of cDNA, RNAi or CRISPR libraries
Storage and Content
1 UnitSDS, CoA, and Instructions
Safety Data Sheets (SDS)
SDS not applicable
Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
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Nucleofector™ Technology – Guideline for Easy Set Up of RNAi Screening Experiments
This guideline aims to help researchers in setting up a successful RNAiscreening experiment using the Nucleofector™ 96-well Shuttle™ System. The recommendations are based on our experience gained in using theNucleofector™ 96-well Shuttle™ System for screens with Thermo ScientificsiRNA libraries in difficult-to-transfect Jurkat T cells and primary HUVECcells2, combined with recommendations collected from the literature. It highlights important parameters that may influence the quality of cellbasedscreening results. -
Designing an RNAi Experiment using Nucleofection® – Technical Reference Guide
The success of an RNAi experiment can be influenced by several parameters. This guide describes the critical steps for setting up an RNAi experiment with siRNA oligos. Partially the guidelines also apply for shRNA. -
Important Vector Factors for Gene Expression – Technical Reference Guide
In this guideline, we show the most important aspects to be considered when choosing an expression vector and their impact on your experimental results. -
4D-Nucleofector® Quick Guide
Quick guide for the first steps of setting up the new 4D-Nucleofector® Units and starting experiment -
4D-Nucleofector® Hardware Manual
Manual for next generation 4D-Nucleofector Core, X, Y, 96-well, and LV Unit Vers.1 -
Essentials for Preparing a Transfection Experiment - Plasmid DNA - Technical Reference Guide
This document will provide you with guidelines on how to prepare quality DNA for transfections. -
Nucleofector® Technology Brochure
Stretching Transfection Dimensions -
How to Use the Cell & Reagent Calculator for the Nucleofector™ 96-well Shuttle™
Lonza_ManualsProductInstructions_How_to_Use_the_Cell__Reagent_Calculator_for_the_Nucleofector_96-well_Shuttle.pdf
Educational Material
Brochures, White Papers etc.
Videos
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Designing an RNAi Experiment using Nucleofection® – Technical Reference Guide
The success of an RNAi experiment can be influenced by several parameters. This guide describes the critical steps for setting up an RNAi experiment with siRNA oligos. Partially the guidelines also apply for shRNA. -
The Nucleofector® 96-well Shuttle System - Nucleofection® of Cell Lines and Primary Cells in an Automated Framework
Lonza WhitePaper -
Important Vector Factors for Gene Expression – Technical Reference Guide
In this guideline, we show the most important aspects to be considered when choosing an expression vector and their impact on your experimental results. -
4D-Nucleofector® Quick Guide
Quick guide for the first steps of setting up the new 4D-Nucleofector® Units and starting experiment -
4D-Nucleofector® Hardware Manual
Manual for next generation 4D-Nucleofector Core, X, Y, 96-well, and LV Unit Vers.1 -
Essentials for Preparing a Transfection Experiment - Plasmid DNA - Technical Reference Guide
This document will provide you with guidelines on how to prepare quality DNA for transfections. -
Cell-based Assays Using Hard-to-transfect Cell Lines and Transiently Transfected Primary Cells
Co-sponsered poster with Promega -
First siRNA Library Screening in Hard-to-Transfect HUVEC and Jurkat Cells
High throughput transfection of siRNA libraries has become a valuable tool in target identifi cation and validation. However, such screenings have so far been constrained to mostly easy-totransfect adherent cell lines. Lonza’s 96-well Shuttle® System, based on the well-established Amaxa® Nucleofector® Technology, extends these approaches to primary and diffi cult-to-transfect cells. -
Efficient Delivery of Dharmacon SMARTpool siRNA Reagents in Hard-To-Transfect Cell Lines Using Amaxa® Nucleofector® 96-well Shuttle System
Lonza WhitePaper
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4D-Nucleofector® Platform - The Story Of
See how breakthrough Nucleofector® Technology innovations in transfection and electroporation culminate in the next generation 4D-Nucleofector® System. -
How it Works - 96-well Shuttle® System (Video Tutorial)
Workflow overview for using the 96-well Shuttle™ System