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Pharma & Biotech

4D-Nucleofector® 96-well Unit

Catalog #: AAF-1003S

The medium-throughput module for the 4D-NucleofectorTM Platform suited for transfection of hard-to-transfect cell lines or primary cells in 96-well format


Product Overview

The 4D-Nucleofector® 96-well Unit is one of the four functional modules of the 4D-Nucleofector® System. The 96-well Unit is a medium-throughput module for the 4D-Nucleofector® System suited for convenient optimization of Nucleofection conditions or as an assay establishment tool.

For integration of the 96-well Unit into a liquid handling workstation we offer a separate Automation Server Software (SBA-3001).

There are cell type-specific Optimized Protocols or recommendations available in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types. 

Technical Specifications

Dimensions: (w × d × h) 24.5 × 10.5 × 28 cm / 9.7 × 4.1 × 11.0 in

Weight: 4.7 kg (10.4 lb)

Power supply: 110 VAC +10%/-20% or 240 VAC +10%/-20% 50–60 Hz, self–regulating

Power consumption: 0 VA (power off) / 20 VA (idle) / 140 VA (busy - during transfection)



The new 96-well Unit is only compatible with the 4D-Nucleofector® Core Unit with part code AAF-1003B


With the introduction of the Next Generation 4D-Nucleofector® System, the 96-well Shuttle® Add-on is no longer available. It is replaced by the new 4D-Nucleofector® 96-well Unit as a fully integrated module for the Next Generation 4D-Nucleofector® Platform. 

Please note: The 96-well Shuttle® Add-on cannot be operated with the Next Generation 4D-Nucleofector® Core Unit and vice versa. The new 96-well Unit cannot be integrated in the 4D-Nucleofector® System of the first generation (Core Unit part code AAF-1001B, or AAF-1002B).



  •  Up to 96 independent programs can be run per plate, processed automatically in <2 minutes 
  • Modular 6 × 16-well Nucleocuvette® Plate for scalable throughput 
  • Fulfills pre-requisites for liquid handling integration


  • Convenient optimization of any difficult-to-transfect cell line in just 1 plate 
  • Variable cell numbers from 104 – 106 cells per reaction 
  • Testing of multiple conditions for assay establishment
  • Screening of cDNA, RNAi or CRISPR libraries


Content & Storage


1 Unit


Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

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Demo Request

Nucleofection in 96-well or 384-well format

Are you interested in seeing the high-throughput Nucleofector™ Technology in your lab and how it performs for your cells? One of our transfection specialists can come to your lab, introduce you to your preferred platform and perform a transfection experiment with your cell type of interest.