text.skipToContent text.skipToNavigation
Need help? Please contact us

Primary Cell Optimization 96-well Nucleofector® Kit

Catalog #: V4SP-9096
For rapid determination of Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-Nucleofector® 96-well Unit.
 2’616.00 CHF
Compare
ST

Product Overview

The Primary Cell Optimization Nucleofector® Kit is the ideal tool to conveniently and rapidly determine Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-Nucleofector® 96-well Unit.

With the unique capability of the 4D-Nucleofector® 96-well Unit to address individual wells of a 96-well Nucleocuvette® Plate with different programs, primary cell optimizations are easily performed within one experiment.

Complete Optimization: Using one 96-well Nucleocuvette® Plate and our pmaxGFPTM Vector as positive control, our five Primary Cell Nucleofector® Solutions P1 – P5 are tested in combination with a pre-selected set of programs plus controls. The remaining 32 wells of the second 96-well Nucleocuvette® Plate can be used for optional fine tuning or performing the first experiment with the selected conditions.

Cell Group-Specific Optimization using Basic Protocols: In addition to a complete optimization described above, we also provide a cell group-specific optimization protocol for some primary cell groups. Those so called Basic Protocols refer to a pre-selected set of 1 or 2 Primary Cell Nucleofector® Solutions and Nucleofector® Programs. Basic Protocols are available for following cell groups:

  • Endothelial cells (mammalian)
  • Epithelial cells (mammalian)
  • Fibroblasts (mammalian)
  • Glial cells (mammalian)
  • Neurons (mammalian)
  • Smooth muscle cells (mammalian)
  • Stem cells (human)

Before doing an optimization for your cell type of interest, please check out our knowledge database for availability of a ready-to-use Lonza Optimized Protocol or any existing data.

Benefits

  • Convenient and rapid determination of optimal Nucleofection conditions for a broad range of primary cells within one experiment
  • Optimal Nucleofection conditions determined on the 4D-Nucleofector® 96-well Unit are transferable to the 4D-Nucleofector® System or the 384-well HT Nucleofector® System

Applications

  • Determination of Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-Nucleofector® 96-well Unit

Content & Storage

Content

0.675 mL Nucleofector® Solution P1

0.675 mL Nucleofector® Solution P2

0.675 mL Nucleofector® Solution P3

0.675 mL Nucleofector® Solution P4

0.675 mL Nucleofector® Solution P5

4 x 0.15 mL Supplement 1

1 x 0.15 mL Supplement 3

2 x 50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0) 

2 x 96-well Nucleocuvette® Plates

Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

Subscribe to our eNewsletter

Keep up to speed on the latest scientific developments, events, tips and tools from Lonza.

Interested in our Webinars?

Need a break from your daily routine? Grab a coffee and check out our free webinars. 

33Teaser_eshop_Knowledge-center_screenshot_4x3.jpg

Knowledge Center

Need technical data?

Browse our Cell and Transfection, Citation, FAQ and Culture Media Technical Databases.