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Pharma & Biotech

Primary Cell Optimization 96-well NucleofectorTM Kit

Catalog #: V4SP-9096
For rapid determination of Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 96-well ShuttleTM Add-On.
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Product Overview

The Primary Cell Optimization 96-well ShuttleTM Kit is the ideal tool to conveniently and rapidly determine Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 96-well ShuttleTM Add-On.

With the unique capability of the 96-well ShuttleTM Add-On to address individual wells of a 96-well NucleocuvetteTM Plate with different programs, primary cell optimizations are easily performed within one experiment.

Complete Optimization: Using one 96-well NucleocuvetteTM Plate and our pmaxGFPTM Vector as positive control, our five Primary Cell NucleofectorTM Solutions P1 – P5 are tested in combination with a pre-selected set of programs plus controls. The remaining 32 wells of the second 96-well NucleocuvetteTM Plate can be used for optional fine tuning or performing the first experiment with the selected conditions.

Cell Group-Specific Optimization using Basic Protocols: In addition to a complete optimization described above, we also provide a cell group-specific optimization protocol for some primary cell groups. Those so called Basic Protocols refer to a pre-selected set of 1 or 2 Primary Cell NucleofectorTM Solutions and NucleofectorTM Programs. Basic Protocols are available for following cell groups:

  • Endothelial cells (mammalian)
  • Epithelial cells (mammalian)
  • Fibroblasts (mammalian)
  • Glial cells (mammalian)
  • Neurons (mammalian)
  • Smooth muscle cells (mammalian)
  • Stem cells (human)

Before doing an optimization for your cell type of interest, please check out our knowledge database for availability of a ready-to-use Lonza Optimized Protocol or any existing data.

Benefits

  • Convenient and rapid determination of optimal Nucleofection conditions for a broad range of primary cells within one experiment
  • Optimal Nucleofection conditions determined on the 96-well ShuttleTM Add-On are transferable to the 4D-NucleofectorTM System or the 384-well HT NucleofectorTM System

Applications

  • Determination of Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 96-well ShuttleTM Add-On

Content & Storage

Content

0.675 mL NucleofectorTM Solution P1

0.675 mL NucleofectorTM Solution P2

0.675 mL NucleofectorTM Solution P3

0.675 mL NucleofectorTM Solution P4

0.675 mL NucleofectorTM Solution P5

4 x 0.15 mL Supplement 1

1 x 0.15 mL Supplement 3

2 x 50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0) 

2 x 96-well NucleocuvetteTM Plates

Instructions

Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

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