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Pharma & Biotech

Cell Line Optimization 96-well NucleofectorTM Kit

Catalog #: V4SC-9096
For rapid determination of Nucleofection conditions for cell lines lacking an Optimized Protocol for the 96-well ShuttleTM Add-On.
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Product Overview

The Cell Line Optimization 96-well ShuttleTM Kit is the ideal tool to conveniently and rapidly determine Nucleofection conditions for cell lines lacking an Optimized Protocol for the 96-well ShuttleTM Add-On.

With the unique capability of the 96-well ShuttleTM Add-On to address individual wells of a 96-well NucleocuvetteTM Plate with different programs, primary cell optimizations are easily performed within one experiment. Using one 96-well NucleocuvetteTM Plate and our pmaxGFPTM Vector as positive control, our three Cell Line NucleofectorTM Solutions SE, SF and SG are tested in combination with a pre-selected set of programs plus controls.

Before doing an optimization for your cell type of interest, please check out our knowledge database for availability of a ready-to-use Lonza Optimized Protocol or any existing data.  

Benefits

  • Convenient and rapid determination of optimal Nucleofection conditions for a broad range of primary cells within one experiment
  • Optimal Nucleofection conditions determined on the 96-well ShuttleTM Add-On are transferable to the 4D-NucleofectorTM System or the 384-well HT NucleofectorTM System

Applications

  • Determination of Nucleofection conditions for cell lines lacking an Optimized Protocol for the 96-well ShuttleTM Add-On

Content & Storage

Content

0.675 mL NucleofectorTM Solution SE

0.675 mL NucleofectorTM Solution SF

0.675 mL NucleofectorTM Solution SG

3 x 0.15 mL Supplement 1

50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)

1 x 96-well NucleocuvetteTM Plate

Instructions

Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

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