4D-Nucleofector® X Unit
4D-Nucleofector® X Unit
Supports Nucleofection® of various cell numbers in 100 µL cuvettes or 20 µL 16-well strips
Product Overview
The 4D-Nucleofector® X Unit is one of the four functional modules of the 4D-Nucleofector® System. It supports Nucleofection of various cell numbers (2 x 104 to 2 x 107) cells in different formats.
There are cell type-specific Optimized Protocols or recommendations available in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
Technical Specifications
Dimensions: (w x h x d) 24.5 × 10.5 × 28 cm / 9.7 × 4.1 × 11.0 in
Weight: 4.7 kg (10.4 lb)
Power: 100 - 240 VAC
Frequency: 50 - 60 Hz
Consumption: 0 VA (power off) / 20 VA (idle) / 140 VA (busy - during transfection)
Fuse: T 2.5A, L250V
Operating temperature: +15°C up to +40°C (59°F - 104°F)
Environment non condensing Voltage limitation: 1500 V
Current limitation: 100 A
Compatibility
This functional module can be operated with all versions of the Core Unit (part code AAF-1001B, AAF-1002B, AAF-1003B)
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Benefits
- Features positions for 20 μL Nucleocuvette® Strips and 100 μL single Nucleocuvette®
- Seamless transfer of conditions between different Nucleofection® Vessels
- Electrically driven drawer for cuvette retainer
Applications
- Supporting Nucleofection of various cell numbers in different formats
- Substrate independent transfection of DNA, RNA, RNP's and proteins
Storage and Content
- 1 Unit
SDS, CoA, and Instructions
Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
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Designing an RNAi Experiment using Nucleofection® – Technical Reference Guide
The success of an RNAi experiment can be influenced by several parameters. This guide describes the critical steps for setting up an RNAi experiment with siRNA oligos. Partially the guidelines also apply for shRNA. -
Important Vector Factors for Gene Expression – Technical Reference Guide
In this guideline, we show the most important aspects to be considered when choosing an expression vector and their impact on your experimental results. -
4D-Nucleofector® Quick Guide
Quick guide for the first steps of setting up the new 4D-Nucleofector® Units and starting experiment -
4D-Nucleofector® Hardware Manual
Manual for next generation 4D-Nucleofector Core, X, Y, 96-well, and LV Unit Vers.1 -
Essentials for Preparing a Transfection Experiment - Plasmid DNA - Technical Reference Guide
This document will provide you with guidelines on how to prepare quality DNA for transfections. -
Nucleofector® Technology Brochure
Stretching Transfection Dimensions
Educational Material
Brochures, White Papers etc.
Videos
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Designing an RNAi Experiment using Nucleofection® – Technical Reference Guide
The success of an RNAi experiment can be influenced by several parameters. This guide describes the critical steps for setting up an RNAi experiment with siRNA oligos. Partially the guidelines also apply for shRNA. -
Important Vector Factors for Gene Expression – Technical Reference Guide
In this guideline, we show the most important aspects to be considered when choosing an expression vector and their impact on your experimental results. -
An Innovative, Highly Efficient Method for Transfecting Large Quantities of Primary Human T-cells
A strong need exists for improved cancer treatment options. Lonza's approach relies on genetically modified, patient-derived cells like T-cells or natural killer cells which express chimeric antigen receptors (CAR) targeting cancer cells. -
4D-Nucleofector® Quick Guide
Quick guide for the first steps of setting up the new 4D-Nucleofector® Units and starting experiment -
4D-Nucleofector® Hardware Manual
Manual for next generation 4D-Nucleofector Core, X, Y, 96-well, and LV Unit Vers.1 -
Essentials for Preparing a Transfection Experiment - Plasmid DNA - Technical Reference Guide
This document will provide you with guidelines on how to prepare quality DNA for transfections. -
Citation List
A list of selected and reviewed publications for the Nucleofector Technology with focus on 4D-Nucleofector System. -
Cell-based Assays Using Hard-to-transfect Cell Lines and Transiently Transfected Primary Cells
Co-sponsered poster with Promega
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4D-Nucleofector® Platform - The Story Of