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4D-Nucleofector® Y Unit

4D-Nucleofector® Y Unit

Catalog #: AAF-1003Y

Enables the direct Nucleofection® of cells in adherence in 24-well culture plates

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Product Overview

 Adherent Nucleofection® Using the Y Unit

Electroporation-based methods have so far required cells to be in suspension for transfection. The Nucleofector® Technology entered a new era and allows direct Nucleofection® Reactions of cells in adherence. Cells which typically grow in adherence in cell culture, can be kept and transfected by Nucleofector® Technology in their physiological state. The Y Unit of the 4D-Nucleofector® System works with disposable conductive polymer Dipping Electrode Arrays that can be inserted into standard 24-well culture plates for Nucleofection® Procedure.
 
The 4D-Nucleofector® Y Unit is one of the four functional modules of the 4D-Nucleofector® System.
There are cell type-specific Optimized Protocols or recommendations available in our knowledge database. In these Optimized Protocols the best Nucleofection® Conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
 

Technical Specifications

Dimensions: (w x h x d): 24.5×10.5×28 cm / 9.7×4.1×11.0
Weight: 5.0 kg (11.0 lb)
Power: 100 - 240 VAC
Frequency 50 - 60 Hz
Consumption: 0 VA (power off) / 20 VA (idle) / 140 VA (busy - during transfection)
Fuse: T 2.5A, L250V
Operating temperature: +15°C up to +40°C (59°F - 104°F)
Environment non condensing
Voltage limitation: 1500 V
Current limitation: 100 A
 

Compatibility

This functional module can be operated with all versions of the Core Unit (part code AAF-1001B, AAF-1002B, AAF-1003B)
 

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Benefits

Pre- and post Nucleofection® culture in 24-well culture plates

  • Nucleofection® of cells at any time point during this culture period, i.e. at a later developmental stage
  • Transfection efficiencies up to 70% combined with high viabilities
  • Compatible with Clonetics® Primary Animal Neurons

Storage and Content

  • 1 Unit
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SDS, CoA, and Instructions

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

  • Designing an RNAi Experiment using Nucleofection® – Technical Reference Guide

    The success of an RNAi experiment can be influenced by several parameters. This guide describes the critical steps for setting up an RNAi experiment with siRNA oligos. Partially the guidelines also apply for shRNA.
  • Important Vector Factors for Gene Expression – Technical Reference Guide

    In this guideline, we show the most important aspects to be considered when choosing an expression vector and their impact on your experimental results.
  • 4D-Nucleofector® Quick Guide

    Quick guide for the first steps of setting up the new 4D-Nucleofector® Units and starting experiment
  • 4D-Nucleofector® Hardware Manual

    Manual for next generation 4D-Nucleofector Core, X, Y, 96-well, and LV Unit Vers.1
  • Essentials for Preparing a Transfection Experiment - Plasmid DNA - Technical Reference Guide

    This document will provide you with guidelines on how to prepare quality DNA for transfections.
  • Nucleofector® Technology Brochure

    Stretching Transfection Dimensions
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Educational Material

Brochures, White Papers etc.

Videos

  • Designing an RNAi Experiment using Nucleofection® – Technical Reference Guide

    The success of an RNAi experiment can be influenced by several parameters. This guide describes the critical steps for setting up an RNAi experiment with siRNA oligos. Partially the guidelines also apply for shRNA.
  • Important Vector Factors for Gene Expression – Technical Reference Guide

    In this guideline, we show the most important aspects to be considered when choosing an expression vector and their impact on your experimental results.
  • 4D-Nucleofector® Quick Guide

    Quick guide for the first steps of setting up the new 4D-Nucleofector® Units and starting experiment
  • 4D-Nucleofector® Hardware Manual

    Manual for next generation 4D-Nucleofector Core, X, Y, 96-well, and LV Unit Vers.1
  • Essentials for Preparing a Transfection Experiment - Plasmid DNA - Technical Reference Guide

    This document will provide you with guidelines on how to prepare quality DNA for transfections.
  • Adherent Nucleofection of Cryopreserved Dissociated Rat Brain Neuronal Cells

    Results for transfecting cultured dissociated rat brain neurons (hippocampal or cortical) using the 4D-Nucleofector Y Unit. It compares results for freshly isolated neurons with Lonza cryopreserved neurons.
  • Citation List

    A list of selected and reviewed publications for the Nucleofector Technology with focus on 4D-Nucleofector System.
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