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Pharma & Biotech

AD2 4D-NucleofectorTM Y Kit

Catalog #: V4YP-2A24
For adherent electroporation of primary cells, e.g. fibroblasts, when working with the 4D-NucleofectorTM Y Unit.
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Product Overview

Electroporation-based methods have so far required cells to be in suspension for transfection. However, the 4D-NucleofectorTM Y Unit allows direct Nucleofection of cells in adherence. Cells which typically grow in adherence in cell culture, can be kept and transfected by Nucleofection in their physiological state. 

The AD2 Primary Cell 4D-NucleofectorTM Y Kit is one of our two kits that are suited for electroporation of primary cells in adherence when working with the 4D-NucleofectorTM Y Unit. The cells are cultured in the 24-well culture plates provided with the kit* and for the transfection step a 24-well Dipping Electrode Array is inserted after replacing culture medium by the specific NucleofectorTM Solution mixed with the DNA or RNA substrate.

This kit has been determined to be optimal for transfecting following cell types:

  • C2C12
  • Fibroblast, cardiac (rat)
  • Fibroblast, dermal, neonatal (human; NHDF-neo)
  • MEF (mouse)

Please refer to the Optimization Protocol for Primary Cells or Cell Lines and transfection results available under "Instructions" or in our knowledge database.

* Other 24-well culture plates may not be compatible with the 24-well Dipping Electrode Array. Please contact our Scientific Support if you want to use alternative plates.

Benefits

  • Nucleofection of cells in adherence without the need for transfer in suspension
  • Transfection efficiencies up to 70% combined with high viabilities

Applications

  • Electroporation of cells in adherence
  • For transfection of 2.5 x 104 - 1 x 105 cells

Content & Storage

Content

2 x 4.5 mL AD2 4D-NucleofectorTM Y Solution
2 x 1 mL Supplement 1
3 x 150 μg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
1 x 24-well Dipping Electrode Array
1 x NunclonTM Δ Surface 24-well plate (Nunc)

Instructions

Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

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