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Irradiated Mouse Embryonic Fibroblasts (iMEF)
Catalog #: M-IFB-482
Cryopreserved ampule of Irradiated Mouse Embryonic Fibroblasts containing ≥2 million cells
Product Overview
Irradiated Mouse primary embryonic fibroblasts (iMEF) are dissociated from day 14 and 15 CD1 mouse embryos, expanded and then inactivated using gamma radiation.
Each vial contains at least 2 million cells. iMEF are convenient and easy to use “feeder cells” commonly co-cultured with embryonic and pluripotent stem cell cultures.
Recommended Media: Lonza's DMEM High Glucose w/ L-Gln, 500 mL (Part 12-604F) containing 10% FBS (Sigma-Aldrich Part F6178)
Benefits
- Ready-To-Use
- No tissue dissection or animal handling required
- Increased data reproducibility
- Characteristic morphology and staining tests confirm tissue origin and cell type
- Negative for mycoplasma, bacteria, yeast and fungi
- Certificate of Analysis (CoA) is available for every batch
Applications
- Serve as “feeder cell” monolayer in embryonic (ES) and induced pluripotent (iPSC) stem cell cultures
- Secrete important growth factors into culture medium to support ES and iPSC undifferentiated state
- Act as cellular matrix to support ES cell growth
Content & Storage
Content
≥ 2 million cryopreserved, gamma irradiated cells per ampule