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Pharma & Biotech

SE Cell Line 4D-NucleofectorTM X Kit L

Catalog #: V4XC-1024
For efficient transfection of difficult-to-transfect cell lines, e.g. Jurkat or MCF-7, in the 4D-NucleofectorTM X Unit (100 µL format).
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Product Overview

The SE Cell Line 4D-NucleofectorTM X Kit L is one of our three 4D-NucleofectorTM Kits that is suited for transfecting difficult-to-transfect cell lines in the 100 µL NucleocuvetteTM Vessel format when working with the 4D-NucleofectorTM X Unit.

This kit has been determined to be optimal for transfecting following cell types:
 

  • 3T3-L1, diff., 3T3-L1, undiff.
  • A-10, A7r5, 
  • BHK-21, BJ
  • C2C12, CaCo-2, Calu-3, Capan-1, Cos-7
  • DU145
  • GH3
  • HCT116, HeLa, HeLa S3
  • IMR-90
  • Jurkat (DSMZ), Jurkat E6.1
  • KG-1a, L6
  • MCF7, MDA-MB-231, MDA-MB-468, MDCK, MDCK II, MEG-01, MG-63, MRC-5
  • NCTC clone 929
  • Panc-1
  • T/G-HA VSMC, T-47D
  • U2OS, U-87 MG
  • WI-38

The respective cell type-specific Optimized Protocols are available in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

The kit is available in different variants for either 12 or 24 reactions.

Benefits

  • High transfection efficiencies in difficult-to-transfect cell lines
  • Cells maintain functionality post transfection
  • Conditions are transferable between 4D-NucleofectorTM System (including 4D-NucleofectorTM including LV Unit), 96-well ShuttleTM System and 384-well NucleofectorTM System

 

Applications

  • Transfection of cell numbers in the range of 2 × 105 to 2 × 107 cells
  • Low throughput applications

Content & Storage

Content

2.25 mL SE Cell Line NucleofectorTM Solution
0.5 mL Supplement 1
50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
24 x 100 µL NucleocuvetteTM Vessel,
24 x Single use pipettes

Instructions

Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

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Need technical data?

Browse our Cell and Transfection, Citation, FAQ and Culture Media Technical Databases.

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