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Pharma & Biotech

SG Cell Line 4D-NucleofectorTM X Kit L

Catalog #: V4XC-3024
For efficient transfection of difficult-to-transfect cell lines, e.g. Ba/F3, Raji, or THP-1, in the 4D-NucleofectorTM X Unit (100 µL format).

Product Overview

The SG Cell Line 4D-NucleofectorTM X Kit L is one of our three 4D-NucleofectorTM Kits that is suited for transfecting difficult-to-transfect cell lines in the 100 µL NucleocuvetteTM Vessel format when working with the 4D-NucleofectorTM X Unit.

This kit has been determined to be optimal for transfecting following cell types:

  • Ba/F3
  • Colo-205
  • H4
  • NIH/3T3
  • Raji, Ramos
  • THP-1
  • WEHI

The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.

The kit is available in different variants for either 12 or 24 reactions.


  • High transfection efficiencies in difficult-to-transfect cell lines
  • Cells maintain functionality post transfection
  • Conditions are transferable between 4D-NucleofectorTM System, 96-well ShuttleTM System and 384-well NucleofectorTM System


  • Transfection of cell numbers in the range of 2 x 105 to 2 x 107 cells
  • Low throughput applications

Content & Storage


2.25 mL SG Cell Line NucleofectorTM Solution
0.5 mL Supplement 1
50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
24 x 100 µL NucleocuvetteTM Vessel
24 x Single use pipettes


Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

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