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Pharma & Biotech

SE Cell Line 4D-NucleofectorTM X Kit S

Catalog #: V4XC-1032
For efficient transfection of difficult-to-transfect cell lines, e.g. Jurkat or MCF-7, in the 4D-NucleofectorTM X Unit (20 µL format).
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Product Overview

The SE Cell Line 4D-NucleofectorTM X Kit S is one of our three 4D-NucleofectorTM Kits that is suited for transfecting difficult-to-transfect cell lines in the 20 µL 16-well NucleocuvetteTM Strip format when working with the 4D-NucleofectorTM X Unit.

This kit has been determined to be optimal for transfecting following cell types:

  • 3T3-L1, diff., 3T3-L1, undiff.
  • A-10, A7r5, 
  • BHK-21, BJ
  • C2C12, CaCo-2, Calu-3, Capan-1, Cos-7
  • DU145
  • GH3
  • HCT116, HeLa, HeLa S3
  • IMR-90
  • Jurkat (DSMZ), Jurkat E6.1
  • KG-1a, L6
  • MCF7, MDA-MB-231, MDA-MB-468, MDCK, MDCK II, MEG-01, MG-63, MRC-5
  • NCTC clone 929
  • Panc-1
  • T/G-HA VSMC, T-47D
  • U2OS, U-87 MG
  • WI-38

The respective cell type-specific Optimized Protocols are available available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

The kit is available in different variants for either 12 or 24 reactions.

Benefits

  • High transfection efficiencies in difficult-to-transfect cell lines
  • Conditions are transferable between 4D NucleofectorTM System, 96-well ShuttleTM System and 384-well NucleofectorTM System

Applications

  • Transfection of low cell numbers in the range of 5 × 104 to 1 × 106 cells
  • Medium throughput applications
  • Optimization of transfection conditions e.g. substrate titrations

Content & Storage

Content

2 x 0.675 mL SE Cell Line NucleofectorTM Solution
2 x 0.15 mL Supplement 1
50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0),
2 x 16-well NucleocuvetteTM Strips (20 μL)

Instructions

Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

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