Nucleofector^TM 2b Device

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Catalog #: AAB-1001

The basic, single cuvette based Nucleofector^TM System for efficient transfection of hard-to-transfect cell lines and primary cells with different substrates (e.g., DNA vectors or siRNA oligonucleotides) in low-throughput format

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Overview

Product Overview

The Nucleofector^TM Device is the single cuvette based system that has been used in research labs since 2001. It allows efficient transfection of hard-to-transfect cell lines and primary cells with different substrates (e.g., DNA vectors or siRNA oligonucleotides) in low-throughput format.

The Nucleofector^TM 2b Device (or older generations I or II) can also be used for bacteria transformation by using alternative cuvettes.

There are cell type-specific Optimized Protocols or recommendations available in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

Technical Specifications

Dimensions (w × d × h): 30 × 23 × 11 cm (11.81 × 9.06 × 4.33 in)
Weight: 2.8 kg (6.2 lb)
Power supply: 100–110 VAC or 230 VAC 50–60 Hz, self-regulating
Power consumption: 50 VA/fuse T630mA L250V
Protection: IP 20, EN 61010-1, UL 61010A-1

Benefits

Highly efficient transfection of primary cells and cell lines
Reliable results due to high viability and preservation of cell functionality
Over 150 ready-to-use Optimized Protocols containing cell type-specific guidance

Applications

Low-throughput transfection in single cuvette format
Transfection of plasmid DNA, siRNA, shRNA, miRNA, RNA and more, e.g., Morpholinos
Transfection of peptides, proteins or small molecules
Approaching 4,000 peer-reviewed publications
Suited for bacteria transformation