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Pharma & Biotech

P3 Primary Cell 384-well Nucleofector™ Kit

Catalog #: V5SP-3010
For high-throughput transfection of primary cells, e.g. human T cells, DCs or CD34, when working with the 384-well HT NucleofectorTM System.

Product Overview

The P3 Primary Cell 384-well NucleofectorTM Kit is one of our five kits suited for high-throughput transfection of primary cells when working with the 384-well HT NucleofectorTM System.

This kit has been determined to be optimal for transfecting following cell types:

  • B cell (human)
  • CD34+ cell (human)
  • Chondrocyte (human)
  • DC (human)
  • DC (mouse), mature
  • ES (human), H9
  • ES (mouse)
  • Hepatocyte (human)
  • HMEC (human)
  • Keratinocyte (human)
  • Macrophage (human)
  • Monocyte (human)
  • Neuron (rat), brain Hi/Cx
  • NHBE (human)
  • RPE (human)
  • T cell (human), stim.
  • T cell (mouse), unstim.

The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

The kit is available in different variants for either 2 x 384 or 10 x 384 reactions.


  • High transfection efficiencies primary cells - Cells maintain functionality post transfection
  • Seamless integration into automated liquid handling environments
  • Conditions are transferable between 4D -ucleofectorTM System, 96-well ShuttleTM System and 384-well NucleofectorTM System


  • Transfection of low cell numbers down to 2 x 104 primary cells
  • High-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR libraries

Content & Storage


90 mL P3 Primary Cell Nucleofector™ Solution, 20 mL Supplement 1, 50 µg pmaxGFP™ Vector (1 μg/μl in 10 mM Tris pH 8.0), 10 384-well Nucleocuvette™ Plate (20 μl)


Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

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