Cell Line Optimization 4D-NucleofectorTM X Kit
The Cell Line Optimization 4D-NucleofectorTM X Kit is the ideal tool to conveniently and rapidly determine Nucleofection conditions for cell lines lacking an Optimized Protocol for the 4D-NucleofectorTM X Unit.
With the unique capability of the 4D-NucleofectorTM X Unit to address individual wells of a 16-well NucleocuvetteTM Strip with different programs, cell line optimizations are easily performed within one experiment. Using three 16-well NucleocuvetteTM Strips and our pmaxGFPTM Vector as positive control, our three Cell Line NucleofectorTM Solutions SE, SF and SG are tested in combination with a pre-selected set of programs plus controls. The remaining 16-well NucleocuvetteTM Strip can be used for optional fine tuning or performing the first experiment with the selected conditions.
Before doing an optimization for your cell type of interest, please check out our knowledge database for availability of a ready-to-use Lonza Optimized Protocol or any existing data.
- Convenient and rapid determination of optimal Nucleofection conditions for a broad range of primary cells within one experiment
- Optimal Nucleofection conditions determined in the 16-well NucleocuvetteTM Strips are transferable to the 100 μL single NucleocuvetteTM in the 4D-NucleofectorTM X Unit
- Conditions are transferable between 4D NucleofectorTM System, 96-well ShuttleTM System and 384-well NucleofectorTM System
- Determination of Nucleofection conditions for cell lines lacking an Optimized Protocol for the 4D-NucleofectorTM X Unit
Content & Storage
0.675 mL Nucleofector™ Solution SE
0.675 mL Nucleofector™ Solution SF
0.675 mL Nucleofector™ Solution SG
3 x 0.15 mL Supplement 1
50 µg pmaxGFP™ Vector (1 μg/μl in 10 mM Tris pH 8.0) ,
4 x 20 µL 16-well Nucleocuvette™ Strips