BEGM™ Bronchial Epithelial Cell Growth Medium BulletKit™
BEGM™ Bronchial Epithelial Cell Growth Medium BulletKit™
Culture system containing BEBMTM Bronchial Epithelial Cell Growth Basal Medium (CC-3171) and BEGMTM Bronchial Epithelial Cell Growth Medium SingleQuotsTM Supplements and Growth Factors (CC-4175).
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Product Overview
BEGMTM Bronchial Epithelial Cell Growth Medium is specially designed to support the growth of human primary bronchial epithelial cells. BEGMTM BulletKitTM is serum-free and has been optimized to grow cells from normal and diseased tissues.
Published research shows BEGMTM BulletKitTM has expanded applications. Our customers have used BEGMTM BulletKitTM to culture well-established cell lines such as BEAS-2B, home-grown bronchial epithelial cells and supporting co-culture models with breast cancer cell lines such as MCF-7 and MDA-MB-231. Refer to the published literature in the 'Data' tab.
BEGMTM is an optimized and readily available media kit providing researchers the confidence and the convenience of a system that works to support their cells. This is especially important for researchers who are utilizing cell lines as a stepping stone to primary cell culture. Working with the same BEGMTM Media between cell lines and primary NHBE can reduce variability within the experiments and simplify the shift for researchers. In addition, if primary cells are utilized as a side-by-side control with normal cell lines or home-grown bronchial epithelial cells, the BEGMTMBulletKitTM Media may also offer cost-benefit since customers can use the same media to support multiple cell types.
Here are publications out of many where our customers have used BEGMTM Growth Media with normal bronchial epithelial cells:
- El Najjar, et al. (2015). PLoSONE 10(2) demonstrates culturing of BEAS-2B bronchial epithelial cell line in BEGMTM Media to examine the effects of cathepsin and furin proteolytic enzymes on viral fusion protein activation in cells
- Hagiwara, et al. (2015). Nature demonstrates BEGMTM Media can ideally support co-culture of Lonza's HUVEC and NHBE in a 3D environment.
- Rider, et al. (2015). PLoS ONE 10(1) used BEGMTM Media to culture primary human bronchial epithelial cells obtained from non-transplanted normal lungs
Purchase our Bronchial Epithelial Cell Culture Kit (catalog#. CC-2540B) and get a discounted price on cells and media. The kit consists of a cryopreserved ampule of bronchial epithelial cells (catalog #CC-2540) and BEGMTM BulletKitTM Growth Media (catalog#. CC-3170).
Request More InfoStorage and Content
1 x BEBMTM Basal Medium (CC-3171), 500 mL
1 x BEGMTM SingleQuotsTM Supplement Pack (CC-4175) containing:
1 x Orange Cap Vial with BPE, 2.00 mL
1 x Lilac Cap Vial with Insulin, 0.50 mL
1 x Natural Cap Vial with Hydrocortisone, 0.50 mL
1 x Red Cap Vial with GA-1000, 0.50 mL
1 x Amber Vial with Retinoic Acid, 0.50 mL
1 x Natural Cap Vial with Transferrin, 0.50 mL
1 x Amber Vial with Triiodothyronine, 0.50 mL
1 x Amber Vial with Epinephrine, 0.50 mL
1 x Green Cap Vial with hEGF, 0.50 mL
SDS, CoA, and Instructions
Safety Data Sheets (SDS)
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Certificate of Analysis (CoA)
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Instructions - Airway Epithelial Cell Systems
Instructions for Proper Use and Culture of Airway Epithelial Cells -
Primary Cells and Media Reference Guide
A comprehensive collection of Lonza primary cell and media products supporting many research areas. -
TechSheet - Bronchial, Tracheal Epithelial Cell Systems
Technical information sheet for Clonetics™ Bronchial and Tracheal cells
Educational Material
Brochures, White Papers etc.
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B-ALI Bronchial Air Liquid Interface Media Kit, a Guaranteed 3D in vitro Model for Respiratory Research
Lonza Whitepaper -
Lonza's Bulletkit™ for Primary Cell Growth
Primary Cells are Different from Cell Lines. Why use the Same Classical Media? Learn how Lonza primary cells and media can support your cell research -
Comparison of Normal and Asthmatic Bronchial Epithelial Cells and Smooth Muscle Cells in Monolayer and RAFT™ 3D Cell Culture System
Understanding the differences between normal and diseased lung cells in 2D vs 3D culture. -
Primary Human Airway Cells and Media
Airway Cell and Media Reference Guide -
Gene Expression Differences in Airway Epithelial Cells from Asthma or COPD Donors
Access to diseased primary cells from patients diagnosed with COPD or asthma provides a convenient, biologically relevant model to assess genetic and in some cases phenotypic changes from normal, healthy donor tissues. In this study, we grew human bronchial epithelial (HBE) cells from normal, COPD-, and asthma-diagnosed human donor lung tissues in an air-liquid interface (ALI) model to assess potential phenotypic changes in vitro as well as provide a differentiated layer of cells to harvest for gene expression studies using the StellARray™ Gene Expression System. -
Primary Cells and Media Reference Guide
A comprehensive collection of Lonza primary cell and media products supporting many research areas. -
Primary Cells for Airway Research – Infographic
Infographic showing how Lonza primary cells can aide in your airway research -
Epithelial Cells Concentration Guide
Epithelial cell culture media guide with SingleQuots® concentration.
Bronchial Epithelial Cells in RAFT™ 3D Cell Culture System
Bronchial Epithelial Cells Grown in BEGM™ BulletKit™ and Cultured in RAFT™ 3D Cell Culture System
Co-culture Experiments with Lonza's Bronchial Epithelial Cells and BEGM™ BulletKit™ Media
Furukawa, et al. demonstrates BEGM™ BulletKit™ is suitable for culturing Lonza's NHBEs as well as to support co-cultures with lung epithelial cell line, BEAS-2B, and breast cancer cell lines, MCF-7 and MDA-MB-231. The breast cancer cell line showed differences in morphology and proliferation rates when cultured with primary NHBE cells vs. BEAS-2B cell line. The paper highlights the importance of using primary NHBE cells as a side-by-side control when conducting experiments with normal lung epithelial cell line such as BEAS-2B because the non-transformed primary cells can validate or offset the data generated using cell lines.