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Pharma & Biotech

4D-Nucleofector® Y Unit

Catalog #: AAF-1003Y

Enables the direct Nucleofection® of cells in adherence in 24-well culture plates

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Product Overview

 Adherent Nucleofection® Using the Y Unit

Electroporation-based methods have so far required cells to be in suspension for transfection. The Nucleofector® Technology entered a new era and allows direct Nucleofection® Reactions of cells in adherence. Cells which typically grow in adherence in cell culture, can be kept and transfected by Nucleofector® Technology in their physiological state. The Y Unit of the 4D-Nucleofector® System works with disposable conductive polymer Dipping Electrode Arrays that can be inserted into standard 24-well culture plates for Nucleofection® Procedure.
 
The 4D-Nucleofector® Y Unit is one of the four functional modules of the 4D-Nucleofector® System.
There are cell type-specific Optimized Protocols or recommendations available in our knowledge database. In these Optimized Protocols the best Nucleofection® Conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
 

Technical Specifications

Dimensions: (w x h x d): 24.5×10.5×28 cm / 9.7×4.1×11.0
Weight: 5.0 kg (11.0 lb)
Power: 100 - 240 VAC
Frequency 50 - 60 Hz
Consumption: 0 VA (power off) / 20 VA (idle) / 140 VA (busy - during transfection)
Fuse: T 2.5A, L250V
Operating temperature: +15°C up to +40°C (59°F - 104°F)
Environment non condensing
Voltage limitation: 1500 V
Current limitation: 100 A
 

Compatibility

This functional module can be operated with all versions of the Core Unit (part code AAF-1001B, AAF-1002B, AAF-1003B)
 

Benefits

 Pre- and post Nucleofection® culture in 24-well culture plates

  • Nucleofection® of cells at any time point during this culture period, i.e. at a later developmental stage
  • Transfection efficiencies up to 70% combined with high viabilities
  • Compatible with Clonetics® Primary Animal Neurons

Content & Storage

Content

1 Unit

Instructions

Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

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On-site Demonstration of 4D-NucleofectorTM Technology

4D-NucleofectorTM X and  Y Unit

Are you interested in seeing the NucleofectorTM Technology for medium-throughput applications in your lab and how it performs for your cells? One of our transfection specialists can come to your lab, introduce you to your preferred platform and perform a transfection experiment with your cell type of interest.