Primary Cell Optimization 96-well Nucleofector™ Kit
What You May Also Need
Product Overview
The Primary Cell Optimization 96-well ShuttleTM Kit is the ideal tool to conveniently and rapidly determine Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 96-well ShuttleTM Add-On.
With the unique capability of the 96-well ShuttleTM Add-On to address individual wells of a 96-well NucleocuvetteTM Plate with different programs, primary cell optimizations are easily performed within one experiment.
Complete Optimization: Using one 96-well NucleocuvetteTM Plate and our pmaxGFPTM Vector as positive control, our five Primary Cell NucleofectorTM Solutions P1 – P5 are tested in combination with a pre-selected set of programs plus controls. The remaining 32 wells of the second 96-well NucleocuvetteTM Plate can be used for optional fine tuning or performing the first experiment with the selected conditions.
Cell Group-Specific Optimization using Basic Protocols: In addition to a complete optimization described above, we also provide a cell group-specific optimization protocol for some primary cell groups. Those so called Basic Protocols refer to a pre-selected set of 1 or 2 Primary Cell NucleofectorTM Solutions and NucleofectorTM Programs. Basic Protocols are available for following cell groups:
- Endothelial cells (mammalian)
- Epithelial cells (mammalian)
- Fibroblasts (mammalian)
- Glial cells (mammalian)
- Neurons (mammalian)
- Smooth muscle cells (mammalian)
- Stem cells (human)
Before doing an optimization for your cell type of interest, please check out our knowledge database for availability of a ready-to-use Lonza Optimized Protocol or any existing data.
Benefits
- Convenient and rapid determination of optimal Nucleofection conditions for a broad range of primary cells within one experiment
- Optimal Nucleofection conditions determined on the 96-well ShuttleTM Add-On are transferable to the 4D-NucleofectorTM System or the 384-well HT NucleofectorTM System
Applications
- Determination of Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 96-well ShuttleTM Add-On
Content & Storage
Content
0.675 mL NucleofectorTM Solution P1
0.675 mL NucleofectorTM Solution P2
0.675 mL NucleofectorTM Solution P3
0.675 mL NucleofectorTM Solution P4
0.675 mL NucleofectorTM Solution P5
4 x 0.15 mL Supplement 1
1 x 0.15 mL Supplement 3
2 x 50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
2 x 96-well NucleocuvetteTM Plates
