P2 Primary Cell 4D-Nucleofector® X Kit L
P2 Primary Cell 4D-Nucleofector® X Kit L
For efficient transfection of specific primary cells, e.g. mouse macrophages and NHDF, in the 4D-Nucleofector® X Unit (100 µL format).
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Product Overview
The P2 Primary Cell 4D-Nucleofector® X Kit L is one of our five 4D-Nucleofector® Kits suited for transfecting primary cells in the 100 µL Nucleocuvette® Vessel format when working with the 4D-Nucleofector® X Unit.
This kit has been determined to be optimal for transfecting following cell types:
- Macrophage (mouse)
- NHDF (human), neonatal
The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
The kit is available in different variants for either 12 or 24 reactions.
Request More InfoBenefits
- High transfection efficiencies for primary cells
- Cells maintain functionality post transfection
- Conditions are transferable between 4D-Nucleofector® System, 96-well Shuttle® System and 384-well Nucleofector® System
Applications
- Transfection of cell numbers in the range of 2 x 105 to 2 x 107 cells
- Low throughput applications
Storage and Content
2 x 0.675 mL P2 Primary Cell Nucleofector® Solution
2 x 0.15 mL Supplement 1
50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
12 x 100 µL Nucleocuvette® Vessel
12 x Single use pipettes
SDS, CoA, and Instructions
Safety Data Sheets (SDS)
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Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
