NHBE – Human Bronchial/Tracheal Epithelial Cells without Retinoic Acid
NHBE – Human Bronchial/Tracheal Epithelial Cells without Retinoic Acid
Cryopreserved ampule of Normal Human Bronchial Epithelial (NHBE) cells without Retinoic Acid containing ≥500,000 cells
Do you need a modified version of this cell product, such as different vialing sizes, or isolations from special donors or species? Contact Lonza CellBio Services to discuss custom isolation, expansion, and testing options for your research
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Product Overview
Normal and Diseased Bronchial Epithelial (NHBE and DHBE) cells are isolated from epithelial lining of airways above bifurcation of the lungs. Lonza's Normal Human Bronchial Epithelial Cells (NHBE) without retinoic acid are available. Certain lots with Small Airway epithelial cells (CC-2547) or normal human lung fibroblasts (CC-2512) from matched donors are available.
Cryopreserved human bronchial epithelial cells without retinoic acid are guaranteed through 15 population doublings. Cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.
Select Applications with Lonza's Human Bronchial Epithelial Cells -
- A Rotating Bioreactor for Scalable Culture and Differentiation of Respiratory Epithelium”. Respiratory epithelium is difficult to grow as it requires a well-maintained polarizing air‐liquid interface (ALI) to maintain differentiation. Raredon et al. describe a scalable and easy method to conduct air-liquid interface with Lonza's bronchial epithelial cells guaranteed for ALI differentiation (Cat. No. CC-2540S).
- Cell concentration and space for growth are required for productive NHBE cell branching in vitro. Click to read this article by Hagiwara et.al that shows how co-cultures with HUVECs in 3D culture influence reconstruction of branched tubular structures in lung epithelial cells.
- Wu, et al. reports for the first time that HBE cells when grown in B-ALITMMedium can differentiate into 3D glandular acinar structures, likesalivary and mammary epithelial. The cells were grown on the basement membrane matrix Matrigel under select conditions and have shown to exhibit formation of brochospheres or tracheospheres with ciliated lining.
Benefits
- All cells test negative for mycoplasma, bacteria, yeast, and fungi
- HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
- Certificate of Analysis provided for every cell lot
Storage and Content
1 x Cryopreserved ampule containing ≥500,000 cells
SDS, CoA, and Instructions
Safety Data Sheets (SDS)
Choose a language to view the SDS.
Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
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Instructions - Airway Epithelial Cell Systems
Instructions for Proper Use and Culture of Airway Epithelial Cells -
Primary Cells and Media Reference Guide
A comprehensive collection of Lonza primary cell and media products supporting many research areas. -
TechSheet - Bronchial, Tracheal Epithelial Cell Systems
Technical information sheet for Clonetics™ Bronchial and Tracheal cells
Educational Material
Brochures, White Papers etc.
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Primary Human Airway Cells and Media
Airway Cell and Media Reference Guide -
Primary Cells and Media Reference Guide
A comprehensive collection of Lonza primary cell and media products supporting many research areas. -
Primary Cells for Airway Research – Infographic
Infographic showing how Lonza primary cells can aide in your airway research
Co-culture Experiments with Lonza's Small Airway Epithelial Cells and SAGM™ BulletKit™ Media
Data Courtesy of Furukawa, et al. Lonza's SAEC cells were co-cultured with primary normal mammary epithelial cells, breast cancer cell lines MCF-7 and MDA-MB-231 in Lonza's SAGM™ BulletKit™ Growth Media. Breast cancer cell lines showed differences in morphology and proliferation rates in the presence of small airway epithelial cells. The image above shows phase contrast imaging with a fluorescent (red) overlay of MCF-7 cell line. MCF-7 cell lines survived as tight clusters in SAGM™ Media. When co-cultured with Lonza's primary SAECs, MCF-7 cells demonstrated enhanced growth all the while staying clustered.
Co-culture Experiments with Lonza's Bronchial Epithelial Cells and BEGM™ BulletKit™ Media
Furukawa, et al. demonstrates BEGM™ BulletKit™ is suitable for culturing Lonza's NHBEs as well as to support co-cultures with lung epithelial cell line, BEAS-2B, and breast cancer cell lines, MCF-7 and MDA-MB-231. The breast cancer cell line showed differences in morphology and proliferation rates when cultured with primary NHBE cells vs. BEAS-2B cell line. The paper highlights the importance of using primary NHBE cells as a side-by-side control when conducting experiments with normal lung epithelial cell line such as BEAS-2B because the non-transformed primary cells can validate or offset the data generated using cell lines.