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CompareHuman Hepatocytes, Cryopreserved, Plateable and Interaction QualifiedCatalog #: HUCPI
Cryopreserved Human Hepatocytes, Plateable and Interaction Qualified are characterized for 3 major mechanisms of drug-drug interactions: transporter activity, enzyme activity, and induction potential (guaranteed to exhibit a 4 fold or greater change in induction activity), providing you with one product to meet more of your hepatocyte DDI study needs. Each ampule contains >5 million cells.
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CompareSample ContainersCatalog #: 80-507L
10 mL depyrogenated glass sample containers with screw caps to be used in applications requiring the endotoxin test. 25/box.
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CompareSample ContainersCatalog #: 80-507U
10 mL glass depyrogenated sample containers with screw caps to be used in applications requiring the endotoxin test. 100/box
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CompareSmBM® Smooth Muscle Cell Growth Basal Medium, 500 mLCatalog #: CC-3181
Basal media required for growth of smooth muscle cells, 500 mL (supplements and growth factors sold separately).
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CompareSeaKem® LE AgaroseCatalog #: 50004SeaKem® LE Agarose, 500 g, ideal for routine analysis of nucleic acids by gel electrophoresis and blotting.Sales unit: each
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CompareSeaKem®, LE AgaroseCatalog #: 50005
SeaKem® LE Agarose, 1 kg, ideal for routine analysis of nucleic acids by gel electrophoresis and blotting.
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CompareSeaKem® LE AgaroseCatalog #: 50002
SeaKem® LE Agarose, 100 g, ideal for routine analysis of nucleic acids by gel electrophoresis and blotting.
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CompareSeaKem® LE AgaroseCatalog #: 50001
SeaKem® LE Agarose, 25 g, ideal for routine analysis of nucleic acids by gel electrophoresis and blotting.
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ComparePyrogen-free Test TubesCatalog #: N205
Pyrogen-free borosilicate glass test tubes without screw-caps. Recommended for gel-clot testing.
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ComparePyrogen-free Dilution TubesCatalog #: N207
Pyrogen-free borosilicate glass dilution tubes to be used in endotoxin testing applications.
Sales unit: each
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TechSheet - Umbilical Vein Endothelial Cell SystemsTechnical information sheet for CloneticsTM HUVEC cells
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Instructions for Use - HUVEC Culturing and Angiogenesis Inhibition Assay using Sartorius Incucyte®Protocol for Angiogenesis assay using HUVEC cells
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Tube Formation Assay with Primary HUVECsIn this study, we have optimized this assay using Lonza's Primary HUVECs and EGM®-2 Media
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Expanded HUVECs for High-throughput ScreeningHUVECs are a good primary endothelial cell type for many in vitro studies and also for several high-throughput screening applications. Consequently, Lonza offers, HUVEC-XL. These cells are expanded to passage-3 from pooled P1-HUVECs and packaged at 10 million cells per ampoule.
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Tube Formation Assay with HUVECHuman Umbilical Vein Endothelial Cells seeded on BME and the formation of endothelial tubes were monitored live with the CytoSMART™ Lux 10X System, the previous version of the CytoSMART™ 2 System. Time-lapse video was recorded for 22 hours at a frame interval of 1 frame every 5 minutes. -
HUVEC-Fibroblast Co-Culture - AngiogenesisTime-Lapse Video of Human Umbilical Vein Endothelial Cell Angiogenic Tube Formation in Co-Culture with Dermal Fibroblasts. Primary Human Umbilical Vein Endothelial Cells (HUVECs) were co-cultured with primary Human Normal Dermal Fibroblast cells at a ratio of 1:3 HUVECs: fibroblasts and a total cell density of 40,000 cells/well in a 96-well plate. Co-cultures were maintained for 5 days with medium changes every 2-3 days in EBMTM-2 medium supplemented with a custom concentration of VEGF (313 pM) to optimize angiogenic tube formation in this setting. Cells were stained with CellTracker® Dye from ThermoFisher. Purple lines indicate angiogenic tube formation detected by the live cell imaging software. At each frame, the software quantifies angiogenesis by counting the number of network branch points (i.e., the number of branches in each network of tubes) per mm2 and the mean network length (i.e., the average of the total network length of tubes per mm2). -
HUVEC-Fibroblast Co-Culture – Angiogenesis Inhibition -
Automated Cell Signaling Assays Using Primary HUVECsLonza's Primary HUVECs are an excellent cell type to measure the effects of pharmacologically-important compounds on vial signaling pathways, and they provide increased data relevancy compared to cell lines.
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Lonza Extends Industry-Standard HUVEC Product LineLonza, the market leader in primary cells, has expanded its human primary cell portfolio with an improved version of Human Umbilical Vein Endothelial Cells (HUVEC) cultured in the absence of additive vascular endothelial growth factor (VEGF).
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Instructions - HUVEC-XL™ Pooled Cell SystemInstructions for culturing Clonetics™ HIVEC-XL™Pooled Cells