Technical Instructions for culturing Clonetics™ Human Fibroblasts Cells

Technical information sheet for Clonetics™ Lung Fibroblast cells

Technical Use and Product Information for CC-2903

Time-Lapse Video of Human Umbilical Vein Endothelial Cell Angiogenic Tube Formation in Co-Culture with Dermal Fibroblasts. Primary Human Umbilical Vein Endothelial Cells (HUVECs) were co-cultured with primary Human Normal Dermal Fibroblast cells at a ratio of 1:3 HUVECs: fibroblasts and a total cell density of 40,000 cells/well in a 96-well plate. Co-cultures were maintained for 5 days with medium changes every 2-3 days in EBMTM-2 medium supplemented with a custom concentration of VEGF (313 pM) to optimize angiogenic tube formation in this setting. Cells were stained with CellTracker® Dye from ThermoFisher. Purple lines indicate angiogenic tube formation detected by the live cell imaging software. At each frame, the software quantifies angiogenesis by counting the number of network branch points (i.e., the number of branches in each network of tubes) per mm2 and the mean network length (i.e., the average of the total network length of tubes per mm2).

M-Fb-481 Mouse Embryonic Fibroblasts MEF - Technical Sheet

Technical information and instructions for CloneticsTM Immortalized Human Dermal Fibroblast cells

Technical information sheet for Clonetics TM Periodontal Ligament Fibroblast cells

Technical information sheet for Clonetics™ Dermal Fibroblast cells systems.

Technical information sheet for Clonetics™ Aortic Adventitial Fibroblasts