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P5 Primary Cell 4D-Nucleofector® X Kit S
Catalog #: V4XP-5032
For efficient transfection of specific primary cells, e.g. HUVEC, in the 4D-Nucleofector® X Unit (20 µL format).
MsGMTM Mesangial Cell Growth Medium BulletKit®
Catalog #: CC-3146
Culture system containing MsGMTM Mesangial Basal Medium (CC-3147) and MsGMTM SingleQuots® Supplements (CC-4146) required for growth of human mesangial cells.
TheraPEAK® Lymphocyte Separation Medium
Catalog #: BP17-829E
Density gradient separation of human lymphocytes
OGMTM Osteoblast Differentiation Medium SingleQuots® Supplements and Growth Factors
Catalog #: CC-4194
OGMTM Osteoblasts Differentiation SingleQuots® Kit
TheraPEAK® SfAAV® Medium
Catalog #: BP12-945Q
TheraPEAK® SfAAV® Medium for AAV production in SF9 cells, chemically defined, hydrolysate free, 1 L
Rat Neonatal Dorsal Root Ganglion (DRG) Neurons
Catalog #: R-DRG-505
Cryopreserved ampule of Rat Neonatal DRG Neurons containing ≥ 200,000 cells
PowerCHO® 2 Serum-free Medium – Chemically Defined in a 10 L bag
Catalog #: BE12-771P10
PowerCHO® 2 CD, with HEPES, Pluronic® F-68, without L-glutamine, phenol red, hypoxanthine or thymidine, 10 L bag
Water for Cell Culture; Bag filled with Water for Cell Culture, bulk packaged sterile filtered WFI, 20L bag GMP
Catalog #: BE02-038P20
Bulk packaged sterile filtered WFI, bag filled with Water for Cell Culture, 20L bag
Mouse CD1 Brain Mixed Astrocytes
Catalog #: M-ASM-430
Cryopreserved ampule of Mouse CD1 Mixed Astrocytes containing ≥ 1 million cells
HepaRG™ Differentiation Medium Supplement
Catalog #: ADD721C
HepaRGTM Differentiation Medium Supplement is used to support the differentiation of the proliferating HepaRGTM101 cells into the mature hepatic-like phenotype.
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Hematopoietic and Immune Cell Products - Essential Tools for Hematopoietic ResearchLonza_Brochures_Hematopoietic_and_Immune_Cell_Products_-_Essential_Tools_for_Hematopoietic_Research.pdf
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References - X-Vivo™Overview of relevant references
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Instructions - Neural Progenitor Cell System, NHNPInstructions for Proper Use
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TechSheet - Neural Progenitor Cell System, NHNPTechnical Product Information Sheet
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Live-cell imaging of CD34+ cells with Nanolive’s 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, in the presence of recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell migration was captured.
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Mitosis of CD34+ cells with Nanolive's 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, supplemented with recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell division was captured. One can distinguish characteristic steps of mitosis affirming that Nanolive’s technology was completely non-invasive.
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Terms and ConditionsCD34+ cells BOGO
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Human CD34+ Cells Technical information and instructions for use
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Transfection Protocol for Human CD34+ Cells - 96-well Shuttle™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell 96-well Nucleofector™ Kit
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Transfection Protocol for Human CD34+ Cells - HT Nucleofector™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell HT-Nucleofector™ Kit
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