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P2 Primary Cell 384-well Nucleofector™ Kit

P2 Primary Cell 384-well Nucleofector™ Kit

Catalog #: V5SP-2002
For high-throughput transfection of primary cells, e.g. mouse macrophages or human fibroblasts, when working with the 384-well HT NucleofectorTM System.
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Product Overview

The P2 Primary Cell 384-well NucleofectorTM Kit is one of our five kits suited for high-throughput transfection of primary cells when working with the 384-well HT NucleofectorTM System.

This kit has been determined to be optimal for transfecting following cell types:

  • Macrophage (mouse)
  • NHDF (human), neonatal

The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

The kit is available in different variants for either 2 x 384 or 10 x 384 reactions.

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Benefits

  • High transfection efficiencies for primary cells
  • Cells maintain functionality post transfection 
  • Seamless integration into automated liquid handling environments
  • Conditions are transferable between 4D-NucleofectorTM System, 96-well ShuttleTM System and 384-well NucleofectorTM System

Applications

  • Transfection of low cell numbers down to 2 x 104 primary cells
  • High-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR libraries

Storage and Content

22.5 mL P2 Primary Cell NucleofectorTM Solution
5 mL Supplement 1
50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
2 x 384-well NucleocuvetteTM Plate (20 μl)

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SDS, CoA, and Instructions

Safety Data Sheets (SDS)

Choose a language to view the SDS.

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

  • Transfection Protocol for NHDF-Neo - HT Nucleofector™ Device

    Instructions of use for transfection of normal human dermal fibroblasts (neonatal) with the P2 Primary Cell HT-Nucleofector™ Kit
  • Basic Transfection Protocol for Fibroblasts - HT Nucleofector™ Device

    Instructions of use for transfection of primary mammalian fibroblast cells with the P2 or P3 Primary Cell HT-Nucleofector™ Kit
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