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Pharma & Biotech

Primary Cell Optimization 4D-NucleofectorTM Y Kit

Catalog #: V4YP-9A48
For rapid determination of Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-NucleofectorTM Y Unit.
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Product Overview

Electroporation-based methods have so far required cells to be in suspension for transfection. However, the 4D-NucleofectorTM Y Unit allows direct Nucleofection of cells in adherence. Cells which typically grow in adherence in culture can be kept and transfected by Nucleofection in their physiological state.

The Primary Cell Optimization 4D-NucleofectorTM Y Kit is suited for rapid determine Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-NucleofectorTM Y Unit. Using one 24-well Dipping Electrode Array and our pmaxGFPTM Vector as positive control, our two Primary Cell NucleofectorTM Solutions for adherent Nucleofection (AD1 and AD2) are tested in combination with a pre-selected set of programs plus controls. The remaining 24-well Dipping Electrode Array can be used for optional fine tuning or performing the first experiment with the selected conditions.

The cells are cultured in the 24-well culture plates provided with the kit*. For the transfection step, a 24-well Dipping Electrode Array is inserted after replacing culture medium by the specific NucleofectorTM Solution mixed with the DNA or RNA substrate.

Before doing an optimization for your cell type of interest, please check out our knowledge database for availability of a ready-to-use Lonza Optimized Protocol or any existing data.

* Other 24-well culture plates may not be compatible with the 24-well Dipping Electrode Array. Please contact our Scientific support if you want to use alternative plates.

Benefits

  • Convenient and rapid determination of optimal conditions for Nucleofection of cells in adherence within one experiment

Applications

  • Determination of Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-NucleofectorTM Y Unit

Content & Storage

Content

2 x 4.5 mL AD1 4D-NucleofectorTM Y Solution

2 x 4.5 mL AD2 4D-NucleofectorTM Y Solution

4 x 1 mL Supplement 1

6 x 150 μg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)

2 x 24-well Dipping Electrode Array

2 x NunclonTM Δ Surface 24-well plate (Nunc)

Instructions

Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

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